Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease with the loss of upper motor neurons in the cortex and lower motor neurons in the brain stem and spinal cord regressively. The vast majority of ALS cases have no familial history are apparently sporadic (SALS), making the modeling of SALS essential to the development of ALS therapeutics. Therefore, human induced pluripotent stem cell (iPSC) from peripheral blood mononuclear cells of a 64-year-old SALS patient were produced using a virus-free protocol and characterized using standard validate methods. This generated iPSC line could be useful to reveal SALS mechanisms and screen drug development.

肌萎缩性侧索硬化症（ALS）是一种神经退行性疾病，其皮质的上运动神经元丢失，脑干和脊髓的下运动神经元逐渐消失。绝大多数无家族史的ALS病例显然是零星的（SALS），这使得SALS的建模对于ALS治疗剂的开发至关重要。因此，使用无病毒方案生产了来自64岁SALS患者外周血单核细胞的人诱导多能干细胞（iPSC），并使用标准验证方法对其进行了表征。生成的iPSC品系可能有助于揭示SALS机制和筛选药物开发。