Cell-penetrating peptides (CPPs), and metal-organic frameworks (MOFs) are promising as next-generation for the delivery of gene-based therapeutic agents. Oligonucleotide (ON)-mediated assembly of nanostructures composed of hierarchical porous zeolitic imidazolate framework (ZIF-8), and nanoparticles such as graphene oxide (GO), and magnetic nanoparticles (MNPs) for gene therapy are reported. Five different types of non-viral vectors (ZIF-8, RhB@ZIF-8, BSA@ZIF-8, MNPs@ZIF-8, and GO@ZIF-8), and three gene therapeutic agents (plasmid, splice correction oligonucleotides (SCO), and small interfering RNA (siRNA)) were investigated. The polyplexes were characterized and applied for gene transfection. The materials show very low toxicity with high efficiency for luciferase transfection. ZIF-8 enhances the transfection of plasmid, SCO, siRNA of CPPs by 2–8 folds. The mechanism of the cell uptakes was also highlighted. Data reveal cell internalization via scavenger class A (SCARA).

细胞穿透肽（CPPs）和金属有机框架（MOFs）有望成为新一代的基于基因的治疗剂。据报道，寡核苷酸（ON）介导的纳米结构的组装由分层的多孔沸石咪唑酸盐骨架（ZIF-8），纳米颗粒（例如氧化石墨烯（GO）和用于基因治疗的磁性纳米颗粒（MNP））组成。五种不同类型的非病毒载体（ZIF-8，RhB @ ZIF-8，BSA @ ZIF-8，MNPs @ ZIF-8和GO @ ZIF-8）和三种基因治疗剂（质粒，剪接校正寡核苷酸） （SCO）和小干扰RNA（siRNA））进行了研究。表征了复合物并应用于基因转染。该材料对荧光素酶的转染显示出非常低的毒性和高效率。ZIF-8可增强质粒SCO的转染，CPP的siRNA降低2–8倍。还强调了细胞摄取的机制。数据显示通过清除剂类A（SCARA）的细胞内在化。