New spiro[indoline-3,4′-[1,3]dithiine]@Cu(NO₃)₂ supported on Fe₃O₄@gly@CE magnetic nanoparticle were synthesized and used as efficient and recyclable catalyst in the synthesis of 2-oxospiro[indoline-3,4′-[1,3]dithiine]-5′-carbonitrile derivatives. The structure of magnetic nanoparticles were confirmed using energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), scanning electron microscopy (SEM), vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA), infrared spectroscopy (FT-IR) and inductively coupled plasma optical emission spectroscopy (ICP‐OES). Subsequently, antibacterial and antifungal activities in terms of inhibition zone diameter, minimum inhibitory concentration, minimum bactericidal concentration and antioxidant activity against the DPPH free radical of the derivatives were investigated. The results revealed acceptable biological effects of the synthetic derivatives and a significant relationship between their structure and biological activity were observed.

Fe ₃ O ₄上负载的新螺[吲哚啉-3,4'-[1,3]二硫代] @Cu（NO ₃）₂合成了@ gly @ CE磁性纳米粒子，并将其用作2-氧代螺并[吲哚啉-3,4'-[1,3]二硫代] -5'-腈衍生物的高效可回收催化剂。使用能量分散X射线光谱仪（EDX），X射线衍射（XRD），扫描电子显微镜（SEM），振动样品磁力计（VSM），热重分析（TGA），红外光谱（ FT-IR）和电感耦合等离子体发射光谱（ICP-OES）。随后，研究了在衍生物的抑制带直径，最小抑制浓度，最小杀菌浓度和对DPPH自由基的抗氧化活性方面的抗菌和抗真菌活性。