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Immunomodulatory activity of low molecular-weight peptides from Nibea japonica skin in cyclophosphamide-induced immunosuppressed mice
Journal of Functional Foods ( IF 5.6 ) Pub Date : 2020-03-13 , DOI: 10.1016/j.jff.2020.103888
Fangmiao Yu , Kang He , Xiaoze Dong , Zhuangwei Zhang , Fenglei Wang , Yunping Tang , Yan Chen , Guofang Ding

In this study, a low molecular weight peptides fraction (<3 kDa, named NJSP) was isolated and purified from the skin protein hydrolysate of Nibea japonica by ultrafiltration. The in vivo immunomodulatory effects of NJSP were investigated in cyclophosphamide (CY)-induced immunosuppressed mice. The intragastric administration of NJSP (100, 200, 400 mg/kg/d) upregulated the immune organ indexes, restored the pathological histomorphology of immune organs, and improved the body weight of CY-treated mice in a time and dose-dependent manner. NJSP treatment also remarkably promoted the proliferation of splenocyte, enhanced phagocytosis of macrophages, restored delayed-type hypersensitivity (DTH). Moreover, NJSP administration dramatically improved hemolysin, tumor necrosis factor α (TNF-α), immunoglobulin (Ig) A, IgG and IgM levels in blood serum in a dose-dependent manner. Besides, NJSP also significantly increased the activities of superoxidase dismutase (SOD) and catalase (CAT), improved the total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) levels in blood serum. These findings confirm that NJSP can protect the immune system against oxidative damage to maintain homeostasis. Finally, NJSP can serve as a promising immunomodulator with potential application in functional food and nutraceutical industry.



中文翻译:

粳米皮肤低分子量肽在环磷酰胺诱导的免疫抑制小鼠中的免疫调节活性

在这项研究中,低分子量的肽级分(<3kDa的,名为NJSP)分离并从皮肤蛋白水解产物纯化日本黄姑鱼通过超滤。在体内在环磷酰胺(CY)诱导的免疫抑制小鼠中研究了NJSP的免疫调节作用。胃内给予NJSP(100、200、400 mg / kg / d)可以上调免疫器官的指标,恢复免疫器官的病理组织形态,并以时间和剂量依赖性方式改善CY处理小鼠的体重。NJSP治疗还显着促进了脾细胞的增殖,增强了巨噬细胞的吞噬作用,恢复了迟发型超敏反应(DTH)。此外,NJSP给药以剂量依赖性方式显着改善血清中的溶血素,肿瘤坏死因子α(TNF-α),免疫球蛋白(Ig)A,IgG和IgM水平。此外,NJSP还显着提高了过氧化物酶歧化酶(SOD)和过氧化氢酶(CAT)的活性,提高了总抗氧化能力(T-AOC),并降低血清丙二醛(MDA)水平。这些发现证实,NJSP可以保护免疫系统免受氧化损伤,从而维持体内平衡。最后,NJSP可以作为有前途的免疫调节剂,在功能性食品和营养食品行业中具有潜在的应用前景。

更新日期:2020-03-16
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