As a member of the reactive nitrogen species (RNS) family, peroxynitrite (ONOO-) as an oxidant and nitrating mediator plays a significant role in some physiopathologic processes. The excessive production of peroxynitrite anion in a drug-damaged liver is a culprit of hepatotoxicity. The detection of peroxynitrite is of vital importance for the treatment of some diseases including cancer and liver injury. In this study, a novel turn-on fluorescent probe IC-ONOO with new chalcone fluorophore was designed and synthesized for the detection of in vitro and in vivo. The probe responded rapidly towards ONOO- (only within 15 min did the fluorescent intensity maximize), and was endowed with high sensitivity and excellent selectivity. Given the fact that the linear correlation between the fluorescent intensity at 560 nm and the concentrations of the probe ranged from 0 to 9 μM, the limit of detection (LOD) was calculated to be 3.1 × 10-8 M. With all the merits, probe IC-ONOO was qualified as a robust tool to monitor peroxynitrite anion under physiopathologic condition. Moreover, it was successfully applied in the imaging of endogenous peroxynitrite in living MCF-7 cells (Human breast carcinoma cells) and mouse drug-damaged liver tissue with low cytotoxicity. Given all the extraordinary merits, great potential has been seen in its application to other peroxynitrite related diseases.

中文翻译：

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具有新型查耳酮荧光团的新型高选择性荧光探针，用于监测和成像活细胞和药物损伤的肝组织中的内源性过氧亚硝酸盐。

作为反应性氮物种（RNS）家族的成员，过氧亚硝酸盐（ONOO-）作为氧化剂和硝化介质在某些生理病理过程中起着重要作用。药物损坏的肝脏中过亚硝酸盐阴离子的过量产生是肝毒性的元凶。过氧亚硝酸盐的检测对于治疗某些疾病（包括癌症和肝损伤）至关重要。在这项研究中，设计并合成了具有新型查尔酮荧光团的新型开启荧光探针IC-ONOO，用于体内外检测。该探针对ONOO-的反应迅速（仅在15分钟内荧光强度达到最大值），并具有高灵敏度和出色的选择性。鉴于560 nm的荧光强度与探针浓度之间的线性相关性介于0到9μM之间，计算得出的检出限（LOD）为3.1×10-8M。探针IC-ONOO被证明是在生理病理条件下监测过亚硝酸盐阴离子的可靠工具。此外，它已成功地用于活的MCF-7细胞（人乳腺癌细胞）和小鼠药物损伤的肝组织中内源性过氧亚硝酸盐的成像，具有低细胞毒性。鉴于所有非凡的优点，将其应用于其他过氧亚硝酸盐相关疾病具有巨大的潜力。探针IC-ONOO被证明是在生理病理条件下监测过亚硝酸盐阴离子的可靠工具。此外，它已成功地用于活的MCF-7细胞（人乳腺癌细胞）和小鼠药物损伤的肝组织中内源性过氧亚硝酸盐的成像，具有低细胞毒性。鉴于所有非凡的优点，将其应用于其他过氧亚硝酸盐相关疾病具有巨大的潜力。探针IC-ONOO被证明是在生理病理条件下监测过亚硝酸盐阴离子的可靠工具。此外，它已成功地用于活的MCF-7细胞（人乳腺癌细胞）和小鼠药物损伤的肝组织中内源性过氧亚硝酸盐的成像，具有低细胞毒性。鉴于所有非凡的优点，将其应用于其他过氧亚硝酸盐相关疾病具有巨大的潜力。