We describe a novel polymer-based zwitterionic stationary phase for hydrophilic interaction chromatography (HILIC). It is prepared by chemical modification of poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) by converting epoxide groups of microsphere surface to diol groups via hydrolysis, then clicking cysteine onto the microsphere with pendant double bonds of microsphere surface via “thiol-ene” click chemistry. The phase has been characterized by scanning electron micrograph, elemental analysis and zeta potential measurement. Diol and zwitterionic group (carboxylate and amine group associated with cysteine) onto the surface of GMA-DVB microspheres make them possess good hydrophilicity, as supported by effective separation towards common polar analytes. It shows good stability at alkaline solution (e.g. pH 10) and negligible bleed (e.g. only 1.7-fold blank and ∼55-fold lower than a commercial silica-based polar phase tested with the minimal bleed level). Such phase exhibits specific separation selectivity to ionic analytes and simultaneous separation of anions and cations is achieved in the retention order of I⁻ < NO₃⁻ < Choline < Br⁻< Cl⁻< K⁺< Na⁺.

我们描述了一种新型的基于聚合物的两性离子固定相，用于亲水相互作用色谱法（HILIC）。它是通过对聚甲基丙烯酸缩水甘油酯-二乙烯基苯（GMA-DVB）进行化学修饰而制备的，方法是通过水解将微球表面的环氧基转化为二醇基，然后通过“硫醇-烯”将半胱氨酸单击到具有微球表面侧挂双键的微球上”点击化学。该相已通过扫描电子显微照片，元素分析和ζ电势测量来表征。GMA-DVB微球表面上的二醇和两性离子基团（与半胱氨酸相关的羧酸根和胺基）使它们具有良好的亲水性，这是通过有效分离常见极性分析物来实现的。在碱性溶液（例如pH 10）下显示出良好的稳定性，而渗出（例如）可忽略不计 仅比空白市售的基于二氧化硅的极性相以最小的渗出水平低1.7倍的空白，低约55倍）。此类相对离子分析物显示出特定的分离选择性，并且阴离子和阳离子的同时分离以I的保留顺序实现^-  <NO ₃ ^-  <胆碱<溴^- <氯^- <K ⁺ <钠⁺。