The extracellular vesicles (EVs) released by plant pathogens of the Pectobacterium genus were investigated. The isolates were obtained using differential centrifugation followed by filtration and were characterized in terms of total protein content and particle size distribution. The transmission electron microscopy (TEM) analysis revealed the presence of two morphologically differentiated subpopulations of vesicles in the obtained isolates. The proteomic analysis using matrix-assisted laser desorption ionization mass spectrometry with time of flight detector (MALDI-TOF/TOF-MS) enabled to identify 62 proteomic markers commonly found in EVs of Gram-negative rods from the Enterobacteriaceae family. Capillary electrophoresis (CE) was proposed as a novel tool for the characterization of EVs. The method allowed for automated and fast (<15 min per sample) separation of vesicles from macromolecular aggregates with low sample consumption (about 10 nL per analysis). The approach required simple background electrolyte (BGE) composed of 50 mM BTP and 75 mM glycine (pH 9.5) and standard UV detection. The report presents a new opportunity for quality control of samples containing EVs.

中文翻译：

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细菌细胞外囊泡的毛细管区带电泳：概念证明。

研究了果胶杆菌属植物病原体释放的细胞外囊泡（EVs）。使用差速离心然后过滤获得分离物，并根据总蛋白含量和粒度分布进行表征。透射电子显微镜（TEM）分析显示，在获得的分离物中存在两个形态上不同的囊泡亚群。使用具有飞行时间检测器（MALDI-TOF / TOF-MS）的基质辅助激光解吸电离质谱技术进行蛋白质组学分析，可以鉴定肠杆菌科的革兰氏阴性杆菌电动车中常见的62种蛋白质组学标记物。毛细管电泳（CE）被提出作为表征电动汽车的新型工具。该方法可实现自动化且快速（< 每个样品15分钟）将囊泡与大分子聚集体分离，样品消耗量低（每次分析约10 nL）。该方法需要由50 mM BTP和75 mM甘氨酸（pH 9.5）组成的简单背景电解质（BGE）和标准UV检测。该报告为包含电动汽车的样品的质量控制提供了新的机会。