Transition metal-doped titanium dioxide nanoparticles (M-TiO₂ NPs) have been studied to enhance the activity of TiO₂ NPs in biomedical applications. In this study, in vitro and in vivo toxicological aspects of M-TiO₂ NPs were reported to assess the safety of these materials. M-TiO₂ NPs were synthesized via a photo-deposition technique. Nickel (Ni) and platinum (Pt) were used as dopants. Physicochemical properties, cytotoxicity, phototoxicity, gene ontology (GO) and dermal toxicity of M-TiO₂ NPs were investigated. Ni-TiO₂ (Ni, 1.02%) and Pt-TiO₂ (Pt, 0.26%) NPs were sphere shape crystals with nanoscale size. ARPE-19 cells were more susceptible to Pt-TiO₂ NPs (EC50, 0.796 mg/mL) than Ni-TiO₂ NPs (EC50, 2.945 mg/mL). M-TiO₂ NPs were rated as probably phototoxic to phototoxic. GO suggested binding function and metabolic processes as a risk mechanism of M-TiO₂ NPs. In vivo toxicological effects of Ni-TiO₂ NPs were not observed on body weight, serum aspartate transaminase/alanine transaminase levels, and skin histology at 61.5–6150 mg/kg. Specifically, skin thickness was not significantly modified (max. 33.2 ± 8.7 μm) and inflammation grade was less than level 2 (max. 1.2 ± 0.4). From these results, Ni-TiO₂ and Pt-TiO₂ NPs show promise as enhanced photocatalysts for safe and sustainable usage.

已经研究了过渡金属掺杂的二氧化钛纳米颗粒（M-TiO ₂ NPs）在生物医学应用中增强TiO ₂ NPs的活性。在这项研究中，据报道M-TiO ₂ NPs的体外和体内毒理学方面评估了这些材料的安全性。通过光沉积技术合成了M-TiO ₂ NP 。镍（Ni）和铂（Pt）用作掺杂剂。研究了M-TiO ₂ NPs的理化性质，细胞毒性，光毒性，基因本体论（GO）和皮肤毒性。Ni-TiO ₂（Ni，1.02％）和Pt-TiO ₂（Pt，0.26％）NP是具有纳米级尺寸的球形晶体。ARPE-19细胞 比Ni-TiO ₂ NPs（EC50，2.945 mg / mL）对Pt-TiO ₂ NPs（EC50，0.796 mg / mL）更敏感。M-TiO ₂ NPs被认为可能是光毒性的。GO建议结合功能和代谢过程作为M-TiO ₂ NPs的危险机制。在体重，血清天冬氨酸转氨酶/丙氨酸转氨酶水平和皮肤组织学为61.5–6150 mg / kg时，未观察到Ni-TiO ₂ NP的体内毒理作用。具体而言，皮肤厚度没有明显改变（最大33.2±8.7μm），并且炎症等级小于2级（最大1.2±0.4）。根据这些结果，Ni-TiO ₂Pt-TiO ₂ NP和Pt-TiO ₂ NPs有望作为增强的光催化剂用于安全和可持续使用。