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Improvement of full-thickness rat skin wounds by photobiomodulation therapy (PBMT): A dosimetric study
Journal of Photochemistry and Photobiology B: Biology ( IF 5.4 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.jphotobiol.2020.111850
Juliana Rodrigues de Castro , Filipi da Silva Pereira , Lin Chen , Victor Elias Arana-Chavez , Rafael Yagüe Ballester , Luisa A. DiPietro , Alyne Simões

Basic dosimetric studies are necessary to support the use of photobiomodulation therapy (PBMT), since the great variety of laser parameters that are reported in the literature have created an obstacle to identifying reproducible results. Thus, the present study evaluates the process of tissue repair after the photobiomodulation therapy, taking into consideration the dose, frequency and the mode of energy delivery used. For this, 6 mm diameter wounds were created on dorsal skin of Wistar rats, and the animals were divided in control and irradiated groups, where L1 and L4 (irradiated with 1 point of 10 J/cm2), L2 and L5 (5 points of 10 J/cm2), L3 and L6 (1 point of 50 J/cm2), respectively for one or multiple days of irradiations. A diode laser, λ 660 nm, 40 mW of power and 0.028 cm2 of spot area was used. Our data showed that the group receiving multiple treatments over the first week post wounding, applied at 10 J/cm2 at each of 5 points on and around the wound (group L5) presented the best improvement of wound closure, higher cytokeratin 10, lower macrophage infiltration, and greater tissue resistance to rupture. We conclude that PBMT improves the skin wound healing process, and the outcomes were directly related to the chosen laser parameters and irradiation mode.



中文翻译:

光生物调节疗法(PBMT)改善全层大鼠皮肤伤口的剂量学研究

基本剂量学研究对于支持光生物调节疗法(PBMT)的使用是必要的,因为文献中报道的各种各样的激光参数已成为鉴定可再现结果的障碍。因此,本研究评估了光生物调节疗法后的组织修复过程,同时考虑了所用剂量,频率和能量传递方式。为此,在Wistar大鼠的背部皮肤上创了6毫米直径的伤口,将动物分为对照组和辐照组,其中L1和L4(辐照1点的10 J / cm 2),L2和L5(5分)。 10 J / cm 2),L3和L6(50 J / cm 2的1点),分别照射一或多天。使用了λ660 nm,功率40 mW和光斑面积0.028 cm 2的二极管激光器。我们的数据表明,该组在伤后第一周接受了多种治疗,并在伤口周围及其周围的5个点分别以10 J / cm2的剂量施用(L5组),其伤口闭合的改善最佳,细胞角蛋白10更高,巨噬细胞更低浸润,更大的组织抗破裂性。我们得出的结论是,PBMT可以改善皮肤伤口的愈合过程,其结果与所选的激光参数和照射模式直接相关。

更新日期:2020-03-16
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