To study the contribution of yeasts to the formation of terpene derivatives during winemaking, a dispersive liquid–liquid microextraction gas chromatography mass spectrometry method was developed for the quantitation of terpenes in white wines, synthetic wine, and a fermented synthetic medium. A mixture of acetone (disperser solvent) and dichloromethane (extraction solvent) was added to 5 mL of sample. The proposed method showed no matrix effect, good linearity in the enological range (from 10 to 200 μg/L), good recovery, and satisfactory inter- and intraday reproducibilities (below 20 and 15% of the relative standard deviation). This sample preparation technique is very interesting for high-throughput studies and economic and environmental reasons because it is fast and easy to operate with high enrichment and consumes a low volume of organic solvents. This method was applied to explore the capacities of 40 yeast strains to produce terpene compounds during fermentation of Chardonnay and Ugni Blanc musts as well as in a synthetic medium. Interestingly, most of the studied compounds were detected and quantified in the resulting wines. This study shows that yeast strains can intrinsically produce terpene derivatives under enological conditions and also highlights the differences between the de novo biosynthesis of terpenes and their precursor-linked production.

中文翻译：

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分散液微萃取技术用于定量葡萄酒中的萜烯。

为了研究酿酒过程中酵母菌对萜烯衍生物形成的贡献，开发了一种分散液-液微萃取气相色谱质谱法，用于定量白葡萄酒，合成酒和发酵合成培养基中的萜烯。将丙酮（分散溶剂）和二氯甲烷（萃取溶剂）的混合物添加到5 mL样品中。所提出的方法没有基质效应，在酶学范围内线性良好（10至200μg/ L），回收率良好，日间和日间重现性令人满意（相对标准偏差在20％和15％以下）。对于高通量研究以及经济和环境原因，这种样品制备技术非常有趣，因为它快速且易于操作，并且富集度高，消耗的有机溶剂量少。该方法被用于探索在霞多丽和Ugni Blanc葡萄的发酵过程中以及在合成培养基中发酵的40种酵母菌株生产萜烯化合物的能力。有趣的是，大多数研究的化合物都在产生的葡萄酒中进行了检测和定量。这项研究表明，酵母菌株可以在酶学条件下固有地产生萜烯衍生物，并突出了两者之间的差异。该方法被用于探索在霞多丽和Ugni Blanc葡萄的发酵过程中以及在合成培养基中发酵的40种酵母菌株生产萜烯化合物的能力。有趣的是，大多数研究的化合物都在产生的葡萄酒中进行了检测和定量。这项研究表明，酵母菌株可以在酶学条件下固有地产生萜烯衍生物，并突出了两者之间的差异。该方法被用于探索在霞多丽和Ugni Blanc葡萄的发酵过程中以及在合成培养基中发酵的40种酵母菌株生产萜烯化合物的能力。有趣的是，大多数研究的化合物都在产生的葡萄酒中进行了检测和定量。这项研究表明，酵母菌株可以在酶学条件下固有地产生萜烯衍生物，并突出了两者之间的差异。萜的从头生物合成及其前体相关产物。