Metal-organic frameworks (MOFs) for enzyme encapsulation-induced biomimetic mineralization under mild reaction conditions are commonly microporous and hydrophobic, which result in a rather high mass transfer resistance of the reactants and restrain the enzyme catalytic activity. Herein, we prepared a type of hierarchical porous and hydrophilic MOF through the biomimetic mineralization of enzymes, zinc ions, 2-methylimidazole, and lithocholic acid. The hierarchical porous structure accelerated the diffusion process of the reactants and the increased hydrophilicity conferred interfacial activity and increased the enzyme catalytic activity. The immobilized enzyme retained higher catalytic activity than the free enzyme and exhibited enhanced resistance to alkaline, organic, and high-temperature conditions. The nanobiocatalyst was reusable and showed long-term storage stability.

中文翻译：

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具有增强的酶活性的分层多孔和亲水性金属有机骨架。

在温和的反应条件下，用于酶促包囊的仿生矿化的金属有机骨架（MOF）通常是微孔和疏水的，这导致反应物的传质性较高，并限制了酶的催化活性。在这里，我们通过酶，锌离子，2-甲基咪唑和硫代胆酸的仿生矿化制备了一种分层的多孔亲水MOF。分层的多孔结构加速了反应物的扩散过程，增加的亲水性赋予了界面活性，并增加了酶的催化活性。固定化酶保留了比游离酶更高的催化活性，并表现出对碱性，有机和高温条件的增强抵抗力。