Abstract Organic compounds stored in sedimentary archives, such as peat bogs, can provide information on numerous processes related to paleoenvironmental changes. The application of multi-proxy methods permits to overcome the limits imposed by reduced sample size, although complex matrices need particular attention to some analytical aspects. Here, we developed and validated a method for the simultaneous determination of 27 n-alkanes, 18 polycyclic aromatic hydrocarbons and 6 sterols in peat by optimizing the extraction and cleanup procedures. The method was evaluated in terms of accuracy, recovery, detection limits and according to green chemistry assessments. In the view of future application in high-resolution multi-proxy geochemical paleoenvironmental studies, it was applied to a test batch of eight samples from the Coltrondo ombrotrophic peatland, located in northern Italy. The multi-proxy approach allowed extracting less than 1 g of dry sample. The stability of proxies over time was assessed by analyzing samples in a wide time and depth range. Results were in agreement with existing historical records and represent the first peat biomarker data for the area.

中文翻译：

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泥炭中多代理生物标志物测定：用于古环境应用的优化提取和净化方法

摘要 储存在沉积档案中的有机化合物，如泥炭沼泽，可以提供与古环境变化相关的众多过程的信息。尽管复杂的矩阵需要特别注意某些分析方面，但多代理方法的应用可以克服样本量减少带来的限制。在这里，我们开发并验证了一种通过优化提取和净化程序同时测定泥炭中 27 种正烷烃、18 种多环芳烃和 6 种甾醇的方法。该方法在准确性、回收率、检测限和绿色化学评估方面进行了评估。从未来在高分辨率多代理地球化学古环境研究中的应用来看，它被应用于来自位于意大利北部的 Coltrondo ombrotrophic 泥炭地的八个样品的测试批次。多代理方法允许提取少于 1 g 的干样品。通过在很宽的时间和深度范围内分析样本来评估代理随时间的稳定性。结果与现有的历史记录一致，代表了该地区的第一个泥炭生物标志物数据。