Perfluorohexane-loaded nanocapsules are interesting materials for many biomedical applications such as oxygen delivery systems or contrast agents. However, their formulation into stable colloidal systems is challenging because of their hydro- and lipophobicity, high density and high vapour pressure. In this study, perfluorohexane-loaded polymeric nanocapsules are prepared for the first time by low-energy emulsification and selective solvent diffusion. The colloidal stability of the perfluorohexane nano-emulsion templates has been improved by the incorporation of an apolar low-density oil (isopropyl myristate) in the dispersed phase, thus addressing droplet coarsening and migration phenomena. The perfluorohexane-loaded nanocapsules prepared from the nano-emulsions show sizes smaller than the corresponding emulsion templates (below 150 nm by dynamic light scattering) and exhibit good stability under storage conditions. Hyperspectral enhanced dark field microscopy revealed a layered core/shell structure and allowed also to confirm the encapsulation of perfluorohexane which was quantified by elemental microanalysis. Although isopropyl myristate has an unfavourable biocompatibility profile, cell viability is enhanced when perfluorohexane is present in the nanocapsules, which is attributed to its high oxygen transport capacity.

装载有全氟己烷的纳米胶囊是许多生物医学应用（例如氧气输送系统或造影剂）中令人感兴趣的材料。然而，由于其疏水性和疏油性，高密度和高蒸气压，将它们配制成稳定的胶体系统具有挑战性。在这项研究中，首次通过低能乳化和选择性溶剂扩散制备了负载全氟己烷的聚合物纳米胶囊。通过在分散相中掺入非极性低密​​度油（肉豆蔻酸异丙酯），可以改善全氟己烷纳米乳液模板的胶体稳定性，从而解决液滴变粗和迁移的现象。由纳米乳液制备的负载全氟己烷的纳米胶囊的尺寸小于相应的乳液模板（通过动态光散射在150 nm以下），并且在储存条件下显示出良好的稳定性。高光谱增强暗场显微镜显示了分层的核/壳结构，并且还可以确认通过元素微分析定量的全氟己烷的包封。尽管肉豆蔻酸异丙酯具有不利的生物相容性特征，但当纳米胶囊中存在全氟己烷时，细胞活力得以增强，这归因于其高的氧传输能力。高光谱增强的暗场显微镜显示了层状的核/壳结构，并且还可以确认通过元素显微分析定量的全氟己烷的包封。尽管肉豆蔻酸异丙酯具有不利的生物相容性特征，但当纳米胶囊中存在全氟己烷时，细胞活力得以增强，这归因于其高的氧传输能力。高光谱增强暗场显微镜显示了分层的核/壳结构，并且还可以确认通过元素微分析定量的全氟己烷的包封。尽管肉豆蔻酸异丙酯具有不利的生物相容性特征，但当纳米胶囊中存在全氟己烷时，细胞活力得以增强，这归因于其高的氧传输能力。