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Simple, Accurate and Precise Determination of the Fungicide Zoxamide in Wine and the Characterization of its Stability in Gastric Conditions by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC)
Analytical Letters ( IF 2 ) Pub Date : 2019-11-23 , DOI: 10.1080/00032719.2019.1695260
Tülay Borahan 1 , Tuğçe Unutkan 2 , Sezgin Bakırdere 1
Affiliation  

Abstract A rapid, accurate and precise method based on reverse-phase high performance liquid chromatography (RP-HPLC) was developed for the trace determination of zoxamide in wine samples. The chromatographic separation was performed at room temperature on a C18 column using mobile phase comprised of methanol and water (82/18, v/v) with a 1.0 mL min−1 flow rate. The analyte was detected at 254 nm. The method was validated based on the accuracy, linearity, precision, and limits of detection and quantification. The linearity of the presented method was found to be in the range from 5.0 µg L−1 to 200 mg L−1 with a correlation coefficient of 0.9999 and a low relative standard deviation (< 10%) for the lowest concentration of the calibration plot, demonstrating suitable precision. The limits of detection and quantification were determined to be 1.9 and 6.2 µg L−1, respectively. The applicability and the accuracy were studied by performing recovery studies on wine samples. The percent recoveries were obtained in the range between 96.7 and 101.5%. The stability and the properties of the analyte were also investigated under simulated gastric conditions. No change in the peak height and area and the retention time of the analyte were observed throughout a 300 min time period.

中文翻译:

使用反相高效液相色谱 (RP-HPLC) 简单、准确和精确测定葡萄酒中的杀菌剂唑沙胺及其在胃部条件下的稳定性

摘要 建立了一种基于反相高效液相色谱(RP-HPLC) 的快速、准确和精密的葡萄酒样品中痕量测定方法。色谱分离在室温下在 C18 柱上使用由甲醇和水 (82/18, v/v) 组成的流动相以 1.0 mL min-1 流速进行。在 254 nm 处检测到分析物。该方法基于准确度、线性、精密度以及检测和定量限进行了验证。发现所提出方法的线性范围为 5.0 µg L-1 至 200 mg L-1,相关系数为 0.9999,校准图最低浓度的相对标准偏差较低 (< 10%) ,显示出合适的精度。检测限和定量限确定为 1。分别为 9 和 6.2 µg L-1。通过对葡萄酒样品进行回收率研究来研究适用性和准确性。获得的百分比回收率介于 96.7% 和 101.5% 之间。还在模拟胃条件下研究了分析物的稳定性和性质。在 300 分钟的时间内,没有观察到峰高和峰面积以及分析物的保留时间发生变化。
更新日期:2019-11-23
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