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Protective effects of Cinnamomum verum, Cinnamomum cassia and cinnamaldehyde against 6-OHDA-induced apoptosis in PC12 cells.
Molecular Biology Reports ( IF 2.8 ) Pub Date : 2020-03-12 , DOI: 10.1007/s11033-020-05284-y
Elham Ramazani 1, 2 , Mahdi YazdFazeli 3 , Seyed Ahmad Emami 4 , Leila Mohtashami 3 , Behjat Javadi 4 , Javad Asili 3 , Zahra Tayarani-Najaran 1, 5
Affiliation  

Cinnamon (Cinnamomum verum and C. cassia) is a medicinal plant, widely-used as a culinary spice. It possesses various therapeutic effects and can slow down the progression of neurological disorders impressively. In this article, the effects of hydro-alcohol extract and essential oil of C. verum and C. cassia and its main bioactive component cinnamaldehyde, has been examined on 6-OHDA-exposed PC12 cells as an in vitro model of Parkinson's disease. The cytotoxicity and cell apoptosis has been induced by 6-OHDA in PC12 cells. The protective effect was determined by measuring cell viability, the amount of reactive oxygen species (ROS), and apoptosis. Cell viability and apoptosis were assessed using resazurin assay, flow cytometry of propidium iodide (PI) stained cells, and western blot analysis. 6-OHDA resulted in the death and apoptosis of cells while, pretreatment with the extract and essential oil of C. verum and C. cassia at 20 µg/ml and cinnamaldehyde at 5 and 10 µM for 24 h could significantly increase the viability (p < 0.001), and decrease ROS content (p < 0.05). Pretreatment with the extracts increased survivin and decreased cyt-c whereas, pretreatment with the essential oil decreased cyt-c, increased survivin, and reduced P-p44/42/p44/42 levels to a level near that of the related control. The extract and essential oil of C. verum and C. cassia can be effective against 6-OHDA cytotoxicity. It is suggested that, the synergistic effects of cinnamaldehyde and other components of extract and essential oil promote cinnamon's medicinal properties.

中文翻译:

肉桂,肉桂和肉桂醛对6-OHDA诱导的PC12细胞凋亡的保护作用。

肉桂(Cinnamomum verum和C. cassia)是一种药用植物,广泛用作烹饪香料。它具有多种治疗作用,可以显着减缓神经系统疾病的进展。在本文中,已经研究了水乙醇提取物和藜,肉桂的精油及其主要生物活性成分肉桂醛对帕金森氏病体外模型对暴露于6-OHDA的PC12细胞的作用。6-OHDA已在PC12细胞中诱导了细胞毒性和细胞凋亡。通过测量细胞活力,活性氧(ROS)和细胞凋亡来确定保护作用。使用刃天青测定,碘化丙啶(PI)染色的细胞的流式细胞仪和蛋白质印迹分析来评估细胞活力和凋亡。6-OHDA导致细胞死亡和细胞凋亡,同时,用20 µg / ml的C. verum和C.决明子的提取物和精油以及5和10 µM的肉桂醛预处理24 h可以显着提高活力(p <0.001),并降低ROS含量(p <0.05)。提取物预处理可增加survivin并降低cyt-c,而精油预处理可降低cyt-c,增加survivin并将P-p44 / 42 / p44 / 42降低至接近相关对照的水平。藜和决明子的提取物和精油可以有效抵抗6-OHDA的细胞毒性。建议肉桂醛与提取物和精油的其他成分的协同作用可提高肉桂的药用性能。20 µg / ml决明子和5和10 µM肉桂醛分别处理24小时可显着提高生存能力(p <0.001),并降低ROS含量(p <0.05)。提取物预处理可增加survivin并降低cyt-c,而精油预处理可降低cyt-c,增加survivin并将P-p44 / 42 / p44 / 42降低至接近相关对照的水平。藜和决明子的提取物和精油可以有效抵抗6-OHDA的细胞毒性。建议肉桂醛与提取物和精油的其他成分的协同作用促进肉桂的药用性能。20 µg / ml决明子和5和10 µM肉桂醛分别处理24小时可显着提高生存能力(p <0.001),并降低ROS含量(p <0.05)。提取物预处理可增加survivin并降低cyt-c,而精油预处理可降低cyt-c,增加survivin并将P-p44 / 42 / p44 / 42降低至接近相关对照的水平。藜和决明子的提取物和精油可以有效抵抗6-OHDA的细胞毒性。建议肉桂醛和提取物与精油的其他成分的协同作用促进肉桂的药用性能。提取物预处理可增加survivin并降低cyt-c,而精油预处理可降低cyt-c,增加survivin并将P-p44 / 42 / p44 / 42降低至接近相关对照的水平。藜和决明子的提取物和精油可以有效抵抗6-OHDA的细胞毒性。建议肉桂醛和提取物与精油的其他成分的协同作用促进肉桂的药用性能。提取物预处理可增加survivin并降低cyt-c,而精油预处理可降低cyt-c,增加survivin并将P-p44 / 42 / p44 / 42降低至接近相关对照的水平。藜和决明子的提取物和精油可以有效抵抗6-OHDA的细胞毒性。建议肉桂醛与提取物和精油的其他成分的协同作用促进肉桂的药用性能。
更新日期:2020-03-12
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