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Antibacterial, Cytocompatible, Sustainably Sourced: Cellulose Membranes with Bifunctional Peptides for Advanced Wound Dressings.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-03-11 , DOI: 10.1002/adhm.201901850 Ramon Weishaupt 1 , Janina N Zünd 1 , Lukas Heuberger 1 , Flavia Zuber 1 , Greta Faccio 1 , Francesco Robotti 2 , Aldo Ferrari 3 , Giuseppino Fortunato 4 , Qun Ren 1 , Katharina Maniura-Weber 1 , Anne Géraldine Guex 1, 4
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-03-11 , DOI: 10.1002/adhm.201901850 Ramon Weishaupt 1 , Janina N Zünd 1 , Lukas Heuberger 1 , Flavia Zuber 1 , Greta Faccio 1 , Francesco Robotti 2 , Aldo Ferrari 3 , Giuseppino Fortunato 4 , Qun Ren 1 , Katharina Maniura-Weber 1 , Anne Géraldine Guex 1, 4
Affiliation
Progressive antibiotic resistance is a serious condition adding to the challenges associated with skin wound treatment, and antibacterial wound dressings with alternatives to antibiotics are urgently needed. Cellulose‐based membranes are increasingly considered as wound dressings, necessitating further functionalization steps. A bifunctional peptide, combining an antimicrobial peptide (AMP) and a cellulose binding peptide (CBP), is designed. AMPs affect bacteria via multiple modes of action, thereby reducing the evolutionary pressure selecting for antibiotic resistance. The bifunctional peptide is successfully immobilized on cellulose membranes of bacterial origin or electrospun fibers of plant‐derived cellulose, with tight control over peptide concentrations (0.2 ± 0.1 to 4.6 ± 1.6 µg mm−2). With this approach, new materials with antibacterial activity against Staphylococcus aureus (log4 reduction) and Pseudomonas aeruginosa (log1 reduction) are developed. Furthermore, membranes are cytocompatible in cultures of human fibroblasts. Additionally, a cell adhesive CBP‐RGD peptide is designed and immobilized on membranes, inducing a 2.2‐fold increased cell spreading compared to pristine cellulose. The versatile concept provides a toolbox for the functionalization of cellulose membranes of different origins and architectures with a broad choice in peptides. Functionalization in tris‐buffered saline avoids further purification steps, allowing for translational research and multiple applications outside the field of wound dressings.
中文翻译:
抗菌,细胞相容性和可持续来源:具有双功能肽的纤维素膜,用于高级伤口敷料。
渐进的抗生素抗性是一种严重的状况,增加了与皮肤伤口治疗相关的挑战,因此迫切需要使用抗生素替代品的抗菌伤口敷料。基于纤维素的膜越来越多地被认为是伤口敷料,因此需要进一步的功能化步骤。设计了一种结合了抗菌肽(AMP)和纤维素结合肽(CBP)的双功能肽。AMP通过多种作用方式影响细菌,从而降低了选择抗生素抗性的进化压力。双功能肽已成功固定在细菌来源的纤维素膜或植物来源的纤维素的电纺丝上,并严格控制肽浓度(0.2±0.1至4.6±1.6 µg mm -2)。通过这种方法,开发了对金黄色葡萄球菌(log4减少)和铜绿假单胞菌(log1减少)具有抗菌活性的新材料。此外,膜在人成纤维细胞的培养物中具有细胞相容性。此外,还设计了一种细胞粘附性CBP-RGD肽并将其固定在膜上,与原始纤维素相比,诱导的细胞扩散增加了2.2倍。通用概念为在肽中有广泛选择的不同来源和结构的纤维素膜功能化提供了一个工具箱。在Tris缓冲盐水中进行功能化可避免进一步的纯化步骤,从而可在伤口敷料领域之外进行转化研究和多种应用。
更新日期:2020-04-08
中文翻译:
抗菌,细胞相容性和可持续来源:具有双功能肽的纤维素膜,用于高级伤口敷料。
渐进的抗生素抗性是一种严重的状况,增加了与皮肤伤口治疗相关的挑战,因此迫切需要使用抗生素替代品的抗菌伤口敷料。基于纤维素的膜越来越多地被认为是伤口敷料,因此需要进一步的功能化步骤。设计了一种结合了抗菌肽(AMP)和纤维素结合肽(CBP)的双功能肽。AMP通过多种作用方式影响细菌,从而降低了选择抗生素抗性的进化压力。双功能肽已成功固定在细菌来源的纤维素膜或植物来源的纤维素的电纺丝上,并严格控制肽浓度(0.2±0.1至4.6±1.6 µg mm -2)。通过这种方法,开发了对金黄色葡萄球菌(log4减少)和铜绿假单胞菌(log1减少)具有抗菌活性的新材料。此外,膜在人成纤维细胞的培养物中具有细胞相容性。此外,还设计了一种细胞粘附性CBP-RGD肽并将其固定在膜上,与原始纤维素相比,诱导的细胞扩散增加了2.2倍。通用概念为在肽中有广泛选择的不同来源和结构的纤维素膜功能化提供了一个工具箱。在Tris缓冲盐水中进行功能化可避免进一步的纯化步骤,从而可在伤口敷料领域之外进行转化研究和多种应用。