Despite excellent processing and biological properties of gelatin for use as a cell carrier, none of the gelatin‐based hydrogel cell carriers reported to date offer all characteristics including quick formation, injectability, self‐healing, and durability, which are simultaneously required for an ideal system. Here, a gelatin‐based hydrogel with dynamic Schiff base linkages, so‐called “dynamic hydrogel,” as an injectable cell carrier consisting of gelatin and amylopectin multiple aldehyde (AMPA), with all the required characteristics is reported. Biocompatibility and osteoinductivity of the hydrogel are verified through the culture of human bone marrow‐derived mesenchymal stem cells (hBMSCs). As live/dead results show, hBMSCs are alive and highly viable ≈85–90% within the hydrogel after 5 days. According to bromodeoxyuridine cell proliferation assay, a significant increase in the number of the cells seeded in the hydrogel confirms its clinical significance for cell therapy. Most importantly, histological visualization using Mason's Trichrome staining indicates secretion of extracellular matrix around the cells loaded in the hydrogel and also expression level evaluation of the crucial osteogenic markers, confirms that the hydrogel can provide osteoinductive support for osteocyte differentiation of hBMSCs after 14 days. Therefore, this hydrogel provides more progress on the path toward bone tissue engineering and further treatment of bone diseases.

中文翻译：

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支链淀粉多醛交联水凝胶作为骨组织工程的可注射和自愈细胞载体

尽管明胶具有出色的加工和生物特性，可作为细胞载体使用，但迄今报道的基于明胶的水凝胶细胞载体均未提供包括快速形成，可注射性，自我修复和耐用性在内的所有特征，这是理想的同时需要的。系统。在这里，报道了具有动态席夫碱键的基于明胶的水凝胶，即所谓的“动态水凝胶”，它是由明胶和支链淀粉多醛（AMPA）组成的可注射细胞载体，具有所有必需的特性。通过人类骨髓间充质干细胞（hBMSCs）的培养验证了水凝胶的生物相容性和骨诱导性。如活/死结果所示，hBMSC在5天后在水凝胶中仍然存活且存活率高达85-90％。根据溴脱氧尿嘧啶核苷细胞增殖测定，植入水凝胶的细胞数量显着增加，证实了其对细胞治疗的临床意义。最重要的是，使用梅森三色（Mason's Trichrome）染色进行的组织学可视化表明水凝胶中所含细胞周围细胞外基质的分泌以及关键成骨标志物的表达水平评估，证实水凝胶可在14天后为hBMSC的骨细胞分化提供骨诱导支持。因此，这种水凝胶为骨组织工程和骨病的进一步治疗提供了更多的进展。s三色染色表明水凝胶中加载的细胞周围有细胞外基质的分泌，并且还评估了重要的成骨标记物的表达水平，证实水凝胶可以为hBMSCs的骨细胞分化提供骨诱导支持。因此，这种水凝胶为骨组织工程和骨病的进一步治疗提供了更多的进展。s三色染色表明水凝胶中加载的细胞周围有细胞外基质的分泌，并且还评估了重要的成骨标记物的表达水平，证实水凝胶可以为hBMSCs的骨细胞分化提供骨诱导支持。因此，这种水凝胶为骨组织工程和骨病的进一步治疗提供了更多的进展。