We demonstrate the potential of an eight-channel light sensing platform system, named Black Box I (BBI), for rapid and highly sensitive measurement of low-level light using a nonradioactive optical readout. We developed, normalized, and characterized the photon sensitivities of the eight channels of the BBI using placental alkaline phosphatase (PLAP) as a model imaging reporter. We found that the BBI system had a statistically strong linear correlation with the reference IVIS Lumina II system. When we applied normalization constants, we were able to optimize the photomultiplier tubes (PMT) of all eight channels of the BBI (up to r² = 0.998). We investigated the biomedical utilities of BBI by: (i) determining alkaline phosphatase activities in mouse plasma samples as a diagnostic secretory biomarker of cancer, and (ii) diagnosing cancer metastases in the organs of mice bearing triple negative breast cancer. We provide an important new addition to low-cost biomedical instruments intended for pre-clinical diagnostic imaging with high sensitivity, high sample throughput, portability, and rapid on-site analysis of low-level light.

中文翻译：

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用于生物医学应用的高灵敏度八通道光感测系统

我们展示了一种名为黑匣子I（BBI）的八通道光感测平台系统在使用非放射性光学读数进行快速且高度灵敏的低光测量方面的潜力。我们使用胎盘碱性磷酸酶（PLAP）作为模型成像报告基因，开发，归一化和表征了BBI八个通道的光子敏感性。我们发现BBI系统与参考IVIS Lumina II系统具有统计上很强的线性相关性。当应用归一化常数时，我们能够优化BBI的所有八个通道的光电倍增管（PMT）（最大r ²= 0.998）。我们通过以下方法研究了BBI的生物医学用途：（i）确定小鼠血浆样品中的碱性磷酸酶活性，作为癌症的诊断性分泌生物标志物，以及（ii）诊断患有三阴性乳腺癌的小鼠器官中的癌症转移。我们为用于临床前诊断成像的低成本生物医学仪器提供了重要的新补充，它们具有高灵敏度，高样品通量，便携性以及对弱光的快速现场分析。