The superoxide anion (O₂˙⁻), with strong oxidizability and nucleophilicity, is the primary reactive oxygen species in living systems, and the overformation of O₂˙⁻ is associated with many physiological and pathological diseases. Therefore, highly sensitive and selective O₂˙⁻ detection is necessary for exploring its roles in disease situations. In this work, we presented a smart ER-targeted two-photon excitation fluorescent O₂˙⁻ probe, named TPER-O₂˙⁻, based on the caged–decaged method of characteristic hydroxyl groups on fluorophores. The TPER-O₂˙⁻ probe, with good sensitivity (detection limit: 0.33 μM) and excellent selectivity for O₂˙⁻, was applied for the detection of endogenous endoplasmic reticulum O₂˙⁻ in HepG2 cells. Furthermore, it was also successfully applied to image the endoplasmic reticulum O₂˙⁻ level fluctuation in an APAP-stimulated liver injury model. The TPER-O₂˙⁻ probe has been demonstrated to be a potential tool to unmask the role of O₂˙⁻ for endoplasmic reticulum stress-relevant clinical diseases.

中文翻译：

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选择性双光子荧光探针在肝损伤模型中内质网超氧阴离子波动的成像

超氧阴离子（O ₂ ˙ ^- ）中，用强氧化和亲核性，是在活的系统中的主要反应性氧物质，以及澳overformation ₂ ˙ ^-与许多生理和病理疾病相关。因此，高灵敏度和选择性O- ₂ ˙ ^-检测是必需的情况下，疾病探索其作用。在这项工作中，我们提出了一个聪明的ER-针对性的双光子激发荧光Ø ₂ ˙ ^-探头，命名为TPER-O ₂ ˙ ^-的基础上，对荧光特征的羟基的笼养decaged方法。该TPER-O₂ ˙ ^-探针，以良好的灵敏度（检测限：0.33μM）和优良的选择性代表O ₂ ˙ ^- ，涂敷了用于检测内源性内质网的O ₂ ˙ ^-在HepG2细胞。此外，也有人成功地应用于图像内质网Ò ₂ ˙ ^-在APAP-刺激肝损伤模型的电平变动。该TPER-O ₂ ˙ ^-探头已被证明是揭露的澳作用的潜在工具₂ ˙ ^-内质网应激相关的临床疾病。