The role of actin fibers in cellular responses to external electric pulses is not clear yet. In this study, we utilized the blocker of actin polymerization, cytochalasin D (cytoD), and investigated its effects on the electropore generation. Eight 100 μs electric pulses of sub-kilovolt per centimeter voltage with 100 ms intervals were applied to adhered cells in vitro, and the membrane permeability was quantified using membrane-impermeable propidium iodide (PI) dye. With cytoD application, the transfer of PI dye decreased significantly in all the applied voltages. At the same time, the roughness of cells increased, the membrane stiffness decreased, and the transmembrane resting potential decreased. Our result supports that actin fibers have clear effects on electroporation through modulating membrane properties including transmembrane resting potential.

肌动蛋白纤维在细胞对外部电脉冲的反应中的作用尚不清楚。在这项研究中，我们利用了肌动蛋白聚合反应的阻断剂，细胞松弛素D（cytoD），并研究了其对电孔生成的影响。将每100毫秒间隔的每千伏电压100 s亚电的8个100 s电脉冲以100毫秒的间隔施加到体外细胞，并使用不可渗透膜的碘化丙啶（PI）染料对膜的渗透性进行定量。随着cytoD的应用，PI染料的转移在所有施加的电压下均显着下降。同时，细胞的粗糙度增加，膜的硬度降低，跨膜静息电位降低。