Abstract In this study, a new electrogenerated chemiluminescence (ECL) reaction is presented through an oxidative-reductive pathway for amisulpride (AMS) as a new coreactant and Ru(bpy)32+ luminophore for the first time. The unique chemical structure of AMS through the presence of electroactive tertiary amino group in combination with the presence bulky electron donating group attached to α‑carbon of its amino group demonstrates the dramatic increase in ECL intensity of luminophore. Upon electrochemical oxidation of both the AMS and Ru(bpy)32+, the electrogenerated AMS+ undergoes spontaneous deprotonation to produce strong reductant that is reacted with electrogenerated Ru(bpy)33+ for excited state production. The developed method presented highly sensitive, selective, rapid, simple and cost-effective ECL approach for AMS detection. Under optimized parameters, the ECL-concentration plot showed a good linear relationship between ECL intensity and AMS concentrations in presence of 1.0 mM Ru(bpy)32+ over the concentration range of (5.0 × 10−10–2.0 × 10−7 M) with a lower detection limit of 7.6 × 10−9 M and a lower quantification limit of 2.51 × 10−8 M. The developed ECL method is successfully utilized for the determination of AMS in Amipride tablets and real human plasma.

中文翻译：

---

Ru(bpy)32+-amisulpride 电化学发光系统的开发用于超灵敏和选择性检测药物和真实血浆中的氨磺必利

摘要 在这项研究中，首次通过氧化还原途径将氨磺必利 (AMS) 作为一种新的共反应物和 Ru(bpy)32+ 发光体提出了一种新的电致化学发光 (ECL) 反应。AMS 独特的化学结构通过电活性叔氨基的存在以及与其氨基的 α-碳相连的庞大给电子基团的存在，证明了发光团的 ECL 强度的显着增加。在对 AMS 和 Ru(bpy)32+ 进行电化学氧化后，电生成的 AMS+ 发生自发去质子化以产生强还原剂，该还原剂与电生成的 Ru(bpy)33+ 反应生成激发态。所开发的方法为 AMS 检测提供了高度灵敏、选择性、快速、简单且经济高效的 ECL 方法。