This report establishes the production and characterization of anthraquinones and phenolic compounds from the adventitious root cultures of Morinda coreia, induced from the leaves on half strength Murashige and Skoog’s (MS) medium fortified with 1.0 mg l⁻¹ of Indole-3 butyric acid (IBA). The in vitro regenerated adventitious roots (AR) were treated with various concentrations of IBA (0.5–5.0 mg l^-1) containing half-strength of liquid MS medium with sucrose (1.5 %). The maximum amount of anthraquinones (149.80 mg g⁻¹ dry weight of AR) and phenolic compounds (71.6 mg g⁻¹ dry weight of AR) were detected on 1.0 mg l⁻¹ and 4.0 mg l⁻¹ of IBA respectively. These proliferated AR on IBA contained 2.10 times more anthraquinones and 1.89 times more phenolics as compared with the yield of in vivo grown roots of M. coreia. Other parameters, such as growth ratio (5.082), fresh weight (1.568 g) and dry weight (0.163 g) of AR were recorded maximum with the concentration of 1.0 mg l⁻¹ IBA. These AR were introduced into the two-stage culture system with various concentrations of chitosan (0.2, 0.4 and 0.8 mg ml^-1) for elicitation. Among these, 0.4 mg ml^-1 chitosan was observed as the best concentration and further used for the production of secondary metabolites (anthraquinones and phenolics) at different time intervals (2–8 days). Growth ratio and biomass (fresh and dry weight) were decreased from second to eighth day of elicitation but the amount of anthraquinones (292.038 mg g^-1 dry weight) and phenolics (86.8 mg g^-1 dry weight) were increased till 4th day of the elicitation. The methods developed in this study could be used for further proliferation of AR and large scale production of anthraquinones and phenolic compounds using in vitro raised leaves of M. coreia.

该报告建立了从巴戟天不定根培养物中蒽醌和酚类化合物的生产和表征，该不定根培养物是在以1.0 mg l ^-1的吲哚3丁酸（IBA ）强化的半强度Murashige和Skoog's（MS）培养基上从叶片诱导而来的。）。的体外再生不定根（AR）用各种浓度的IBA的处理（0.5-5.0毫克升^-1）含有蔗糖（1.5％）的液体MS培养基的半强度。在1.0 mg l ^-1和4.0 mg l ^-1上检测到最大量的蒽醌（149.80 mg g ^-1 AR干重）和酚类化合物（71.6 mg g ^-1 AR干重）分别为IBA。与体内生长的M. coreia根的产量相比，这些在IBA上增殖的AR含有2.10倍的蒽醌和1.89倍的酚类。以1.0 mg ^-1 IBA的浓度记录了其他参数，例如生长比（5.082），鲜重（1.568 g）和干重（0.163 g）最大。将这些AR与各种浓度的壳聚糖（0.2、0.4和0.8 mg ml ^-1）一起引入两阶段培养系统中进行诱导。其中0.4 mg ml ^-1壳聚糖被认为是最佳浓度，并在不同的时间间隔（2-8天）内用于次生代谢产物（蒽醌和酚类）的生产。从诱变的第二天到第八天，其生长率和生物量（鲜重和干重）降低，但是直到第4天，蒽醌（292.038 mg g ^-1干重）和酚类（86.8 mg g ^-1干重）的量增加。启发。本研究开发的方法可用于进一步扩增AR，并用体外培养的M. coreia叶片大量生产蒽醌和酚类化合物。