Abstract Acetylcholine (ACh) is a neurotransmitter which plays central role in transmitting information between nerve - skeletal intersection and neurons present in the spinal cord. Presently, there are few conventional techniques for the detection of ACh however they have certain limitations including requirement of trained technicians, time consumption and low sensitivity. Therefore, it is necessary to develop new methods based on emerging nanotechnology for detecting ACh in real samples. The ACh biosensor was fabricated with the co-immobilization of acetylcholinesterase (AChE) and choline oxidase (ChO) over the Au-electrode coated with nanocomposite layer of multi-walled carbon nanotubes (MWCNT)-manganese oxide (MnO2)- reduced graphene oxide (rGO). Characterizations of the nanocomposites were accomplished by Scanning Electron Microscopy (SEM). The cyclic voltammetry (CV) technique was adopted for the testing and optimization of the developed biosensor. The observation for current in proportion to various ACh concentrations show a linear relation ranging between 0.1–100 μM. The detection limit (LOD) of the biosensor was obtained up to 0.1 μM.

中文翻译：

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神经递质的灵敏生物传感：二维材料包裹的纳米管和二氧化锰复合材料用于检测乙酰胆碱

摘要 乙酰胆碱 (ACh) 是一种神经递质，在神经-骨骼交叉点和脊髓中的神经元之间传递信息中起核心作用。目前，用于检测乙酰胆碱的常规技术很少，但它们具有一定的局限性，包括需要经过培训的技术人员、耗时和灵敏度低。因此，有必要开发基于新兴纳米技术的新方法来检测实际样品中的乙酰胆碱。ACh 生物传感器是通过将乙酰胆碱酯酶 (AChE) 和胆碱氧化酶 (ChO) 共同固定在涂有多壁碳纳米管 (MWCNT)-氧化锰 (MnO2)-还原氧化石墨烯纳米复合层的 Au 电极上制成的。 rGO）。纳米复合材料的表征是通过扫描电子显微镜 (SEM) 完成的。采用循环伏安法 (CV) 技术对开发的生物传感器进行测试和优化。电流与各种 ACh 浓度成正比的观察结果显示出介于 0.1-100 μM 之间的线性关系。生物传感器的检测限 (LOD) 高达 0.1 μM。