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Comparative expression profiles of host circulating miRNAs in response to Trichinella spiralis infection.
Veterinary Research ( IF 4.4 ) Pub Date : 2020-03-11 , DOI: 10.1186/s13567-020-00758-0
Xiao Han Ma 1, 2 , Hui Jun Ren 1, 2 , Ruo Yu Peng 1, 2 , Yi Li 1, 2 , Liang Ming 1, 2
Affiliation  

Trichinellosis is an important food-borne parasitic zoonosis throughout the world. At present, the mechanisms of Trichinella spiralis infection remain unclear. Acquiring detailed information on the host-Trichinella interaction would be beneficial for the development of new strategies for trichinellosis control. Circulating miRNAs are stably detectable in the blood of humans and animals infected with parasites. Circulating miRNAs might regulate the expression of target genes in pathological responses during infection and might be novel potential biomarkers of parasitic diseases. In the present study, a total of ten differentially expressed circulating mouse miRNAs with |log2(fold change)| ≥ 1.0 and FDR < 0.01 were found during T. spiralis infection, of which five were upregulated and five were downregulated. GO and KEGG analyses showed that the target genes of the ten miRNAs were enriched in many signalling pathways, especially focal adhesion, MAPK pathway, and so on. The results of qRT-PCR showed that among the five upregulated miRNAs, mmu-miR-467a-3p and mmu-miR-467d-3p expression in mouse serum reached a peak at 30 days post-infection (dpi). The expression of mmu-miR-376b-3p and mmu-miR-664-3p increased significantly at 18 dpi and then decreased at 30 dpi. The expression of mmu-miR-292a-5p gradually decreased from 12 to 30 dpi. Among the 5 downregulated miRNAs, mmu-miR-199a-5p expression was significantly downregulated at 30 dpi, while the expression levels of the other four miRNAs (mmu-miR-455-5p, mmu-miR-125b-5p, mmu-miR-125a-5p, and mmu-miR-615-3p) were significantly lower compared with the control, showing a steady downregulation at different phases of infection. These findings will help to further understand the host-Trichinella interaction and provide promising serum biomarkers for trichinellosis.

中文翻译:

响应旋毛虫感染的宿主循环miRNA的比较表达谱。

旋毛虫病是全世界重要的食源性寄生虫人畜共患病。目前,旋毛虫感染的机制尚不清楚。获取有关宿主-旋毛虫相互作用的详细信息将有助于开发旋毛虫病控制新策略。在感染了寄生虫的人和动物的血液中可以稳定地检测到循环中的miRNA。循环中的miRNA可能会调节感染过程中病理反应中靶基因的表达,并且可能是寄生虫疾病的新型潜在生物标记。在本研究中,共有十个差异表达的循环小鼠miRNA,其| log2(倍数变化)| 在螺旋藻感染期间发现≥1.0并且FDR <0.01,其中五个被上调,五个被下调。GO和KEGG分析表明,十个miRNA的靶基因在许多信号传导途径中富集,尤其是粘着斑粘附,MAPK途径等。qRT-PCR的结果表明,在五个上调的miRNA中,小鼠血清中的mmu-miR-467a-3p和mmu-miR-467d-3p表达在感染后30天(dpi)达到峰值。mmu-miR-376b-3p和mmu-miR-664-3p的表达在18 dpi时显着增加,然后在30 dpi时降低。mmu-miR-292a-5p的表达从12 dpi逐渐降低到30 dpi。在5个下调的miRNA中,mmu-miR-199a-5p的表达在30 dpi时显着下调,而其他四个miRNA(mmu-miR-455-5p,mmu-miR-125b-5p,mmu-miR -125a-5p和mmu-miR-615-3p)与对照组相比明显降低,在感染的不同阶段显示出稳定的下调。这些发现将有助于进一步了解宿主-旋毛虫的相互作用,并为旋毛虫病提供有希望的血清生物标志物。
更新日期:2020-04-22
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