BACKGROUND Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA-induced silencing complex. METHODS Microarray was conducted on three groups of tumor tissues and normal tissues from patients with GCTB, and results showed different expression profiles of miRNAs with Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis. The functions of miR-187-5p and miR-1323, which were highly expressed in GCTB, were examined by 5-ethynyl-2'-deoxyuridine (EDU), transwell, and CCK8 assays. RNAhybrid et al. (RNA prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments. RESULTS miR-187-5p and miR-1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Luciferase assay confirmed that the two microRNAs can bind to the 3' untranslated regions (UTR) of mRNA of FRS2. And, rescue experiments verified the relationships between the two microRNAs and FRS2. CONCLUSION There were some different-expressed microRNAs between GCTB and normal tissues. miR-187-5p and miR-1323 can regulate the biological functions of GCTB through influencing the expression of FRS2.

中文翻译：

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miRNA在骨巨细胞瘤中的表达谱显示miR-187-5p和miR-1323可通过抑制FRS2来调节生物学功能。

背景技术骨巨细胞瘤（GCTB）被认为是一种边缘性肿瘤，具有复发和易位的趋势。微小RNA是一种小型非编码RNA，可通过RNA诱导的沉默复合体抑制目标mRNA的翻译。方法对来自GCTB患者的三组肿瘤组织和正常组织进行基因芯片分析，结果显示，通过基因本体分析和《京都议定书》及《基因组与基因组百科全书》分析，miRNA的表达谱不同。通过5-乙炔基-2'-脱氧尿苷（EDU），transwell和CCK8分析检测了在GCTB中高度表达的miR-187-5p和miR-1323的功能。RNAhybrid等。（RNA预测软件）预测这两个microRNA靶向成纤维细胞生长因子受体底物2（FRS2），萤光素酶测定和救援实验对此进行了验证。结果miR-187-5p和miR-1323在肿瘤组织中高表达。它们可以在体外共同调节GCTB的生物学功能。萤光素酶测定证实，这两个microRNA可以结合FRS2 mRNA的3'非翻译区（UTR）。并且，救援实验验证了这两个microRNA与FRS2之间的关系。结论GCTB与正常组织之间存在一些不同表达的microRNA。miR-187-5p和miR-1323可以通过影响FRS2的表达来调节GCTB的生物学功能。FRS2 mRNA的非翻译区（UTR）。并且，救援实验验证了这两个microRNA与FRS2之间的关系。结论GCTB与正常组织之间存在一些不同表达的microRNA。miR-187-5p和miR-1323可以通过影响FRS2的表达来调节GCTB的生物学功能。FRS2 mRNA的非翻译区（UTR）。并且，救援实验验证了这两个microRNA与FRS2之间的关系。结论GCTB与正常组织之间存在一些不同表达的microRNA。miR-187-5p和miR-1323可以通过影响FRS2的表达来调节GCTB的生物学功能。