Considering the high abundance of spliced RNAs in testis compared to other tissues, it is needed to construct the landscape of alternative splicing during spermatogenesis. However, there is still a lack of the systematic analysis of alternative RNA splicing in spermatogenesis. Here, we constructed a landscape of alternative RNA splicing during mouse spermatogenesis based on integrated RNA-seq data sets. Our results presented several novel alternatively spliced genes (Eif2s3y, Erdr1 Uty and Zfy1) in the Y chromosome with a specific expression pattern. Remarkably, the alternative splicing genes were grouped into co-expression networks involved in the microtubule cytoskeleton organization and post-transcriptional regulation of the gene expression, indicating the potential pathway to germ cell generation. Furthermore, based on the co-expression networks, we identified Atxn2l as a potential key gene in spermatogenesis, which presented dynamic expression patterns in different alternative splicing types. Ultimately, we proposed splicing regulatory networks for understanding novel and innovative alternative splicing regulation mechanisms during spermatogenesis. In summary, our research provides a systematic analysis of alternative RNA splicing and some novel spliced genes related to spermatogenesis.

中文翻译：

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精子发生过程中小鼠基因的特异性表达和可变剪接。

考虑到与其他组织相比，睾丸中剪接RNA的丰度很高，因此需要在精子发生过程中构建替代剪接的格局。但是，仍然缺乏对精子发生过程中可替代的RNA剪接的系统分析。在这里，我们基于整合的RNA-seq数据集构建了小鼠精子发生过程中替代性RNA剪接的格局。我们的结果提出了几个新颖的选择性剪接基因（Eif2s3y，Erdr1 Uty和Zfy1）在Y染色体上具有特定的表达模式。值得注意的是，其他剪接基因被分为参与微管细胞骨架组织和基因表达的转录后调控的共表达网络，表明了生殖细胞生成的潜在途径。此外，基于共表达网络，我们确定Atxn21为精子发生中的潜在关键基因，其在不同的可变剪接类型中表现出动态表达模式。最终，我们提出了剪接调控网络，以了解精子发生过程中新颖和创新的替代剪接调控机制。总而言之，我们的研究为替代性的RNA剪接和一些与精子发生有关的新型剪接基因提供了系统的分析。