Actin and microtubule filaments, with their auxiliary proteins, enable the cytoskeleton to carry out vital processes in the cell by tuning the organizational and mechanical properties of the network. Despite their critical importance and interactions in cells, we are only beginning to uncover information about the composite network. The challenge is due to the high complexity of combining actin, microtubules, and their hundreds of known associated proteins. Here, we use fluorescence microscopy, fluctuation, and cross-correlation analysis to examine the role of actin and microtubules in the presence of an antiparallel microtubule crosslinker, MAP65, and a generic, strong actin crosslinker, biotin–NeutrAvidin. For a fixed ratio of actin and microtubule filaments, we vary the amount of each crosslinker and measure the organization and fluctuations of the filaments. We find that the microtubule crosslinker plays the principle role in the organization of the system, while, actin crosslinking dictates the mobility of the filaments. We have previously demonstrated that the fluctuations of filaments are related to the mechanics, implying that actin crosslinking controls the mechanical properties of the network, independent of the microtubule-driven re-organization.

中文翻译：

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肌动蛋白和微管交联剂可调节迁移率并控制复合细胞骨架网络中的共定位。

肌动蛋白和微管细丝及其辅助蛋白使细胞骨架能够通过调节网络的组织和机械特性，在细胞中执行重要过程。尽管它们在细胞中具有至关重要的作用和相互作用，但我们才刚刚开始发现有关复合网络的信息。挑战是由于肌动蛋白，微管及其数百种已知相关蛋白结合的高度复杂性。在这里，我们使用荧光显微镜，波动和互相关分析来检查肌动蛋白和微管在反平行微管交联剂MAP65和通用强肌动蛋白交联剂生物素– NeutrAvidin存在下的作用。对于固定比例的肌动蛋白丝和微管丝，我们改变每种交联剂的量，并测量长丝的组织和波动。我们发现微管交联剂在系统的组织中起主要作用，而肌动蛋白的交联则决定了细丝的流动性。先前我们已经证明，细丝的波动与力学有关，这意味着肌动蛋白交联可控制网络的机械性能，而与微管驱动的重组无关。