Two tridentate ligand systems bearing N-heterocyclic carbene (NHC), amine and carboxylate donor groups coupled to benzothiazole- or stilbene-based amyloid binding moieties were synthesised. Reaction of the imidazolium salt containing pro-ligands with Re(CO)₅Cl yielded the corresponding rhenium metal complexes which were characterised by NMR, and X-ray crystallography. These ligands are of interest for the potential preparation of technetium-99m imaging agents for Alzheimer's disease and the capacity of these rhenium complexes bind to amyloid fibrils composed of amyloid-β peptide and amyloid plaques in human frontal cortex brain tissue was evaluated using fluorescence microscopy. These studies show that the complexes bound efficiently to amyloid-β fibrils and some evidence of binding to amyloid-β plaques.

中文翻译：

---

带电荷的中性三齿N-杂环卡宾配体的中性三羰基配合物，可与阿尔茨海默氏病的淀粉样斑块结合

合成了两个带有N-杂环卡宾（NHC），胺和羧酸酯供体基团的三齿配体系统，这些基团与基于苯并噻唑或的淀粉样蛋白结合部分偶联。含原配体的咪唑鎓盐与Re（CO）₅ Cl的反应产生相应的rh金属配合物，其通过NMR和X射线晶体学表征。这些配体对于潜在制备用于阿尔茨海默氏病的tech 99m成像剂具有重要意义，并且使用荧光显微镜评估了这些rh络合物与人额叶皮质脑组织中由淀粉样β-肽和淀粉样斑块组成的淀粉样原纤维结合的能力。这些研究表明，该复合物与淀粉样蛋白β原纤维有效结合，并且有与淀粉样蛋白β斑块结合的证据。