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Global mistranslation increases cell survival under stress in Escherichia coli.
PLOS Genetics ( IF 4.5 ) Pub Date : 2020-03-09 , DOI: 10.1371/journal.pgen.1008654
Laasya Samhita 1 , Parth K Raval 1 , Deepa Agashe 1
Affiliation  

Mistranslation is typically deleterious for cells, although specific mistranslated proteins can confer a short-term benefit in a particular environment. However, given its large overall cost, the prevalence of high global mistranslation rates remains puzzling. Altering basal mistranslation levels of Escherichia coli in several ways, we show that generalized mistranslation enhances early survival under DNA damage, by rapidly activating the SOS response. Mistranslating cells maintain larger populations after exposure to DNA damage, and thus have a higher probability of sampling critical beneficial mutations. Both basal and artificially increased mistranslation increase the number of cells that are phenotypically tolerant and genetically resistant under DNA damage; they also enhance survival at high temperature. In contrast, decreasing the normal basal mistranslation rate reduces cell survival. This wide-ranging stress resistance relies on Lon protease, which is revealed as a key effector that induces the SOS response in addition to alleviating proteotoxic stress. The new links between error-prone protein synthesis, DNA damage, and generalised stress resistance indicate surprising coordination between intracellular stress responses and suggest a novel hypothesis to explain high global mistranslation rates.



中文翻译:

全局错误翻译会增加大肠杆菌在压力下的细胞存活率。

错误翻译通常对细胞有害,尽管特定的错误翻译的蛋白质可在特定环境中带来短期利益。但是,鉴于其总体成本高昂,全球误译率很高的普遍性仍然令人困惑。改变大肠杆菌的基础错译水平通过几种方式,我们表明,广泛的错误翻译可通过快速激活SOS反应来提高DNA损伤下的早期存活率。错误翻译的细胞在暴露于DNA损伤后仍能维持较大的种群,因此采样关键有益突变的可能性更高。基础和人为增加的错误翻译都会增加在DNA损伤下具有表型耐受性和遗传抗性的细胞数量。它们还提高了高温下的存活率。相反,降低正常的基底错误翻译率会降低细胞存活率。这种广泛的抗逆性依赖于Lon蛋白酶,Lon蛋白酶被证明是除减轻蛋白毒性应激外还诱导SOS反应的关键效应物。容易出错的蛋白质合成,DNA损伤,

更新日期:2020-04-06
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