The collaborative endeavor in tissue engineering is to fabricate a bio-mimetic extracellular matrix to assist tissue regeneration. Thus, a novel injectable tissue scaffold was fabricated by exploring nanotailored hyaluronic acid (nHA) and methylcellulose (MC) (nHAMC) along with pristine HA based MC scaffold (HAMC). nHA with particle size ∼22 ± 5.3 nm were obtained and nHAMC displayed a honeycomb-like 3D microporous architecture. Nano-HA bestowed better gel strength, physico-rheological and biological properties than HA. It creditably reduced the high content of salt to reduce the gelation temperature of MC. The properties ameliorated with increased in-corporation of nano-HA. The addition of salt showed more prominent effect on gelation temperature of nHAMC than in HAMC; and salting-out effect was dependent on nHA/HA content. Biocompatible nHAMC assisted adequate cell adherence and proliferation with more extended protrusions with better migration rate than control. Thus, biomodulatory effect of nanotailored glycosaminoglycan could be asserted to design an efficient thermo-responsive scaffold.

组织工程领域的合作努力是制造仿生细胞外基质以帮助组织再生。因此，通过探索纳米定制透明质酸 (nHA) 和甲基纤维素 (MC) (nHAMC) 以及原始 HA 基 MC 支架 (HAMC)，制造了一种新型可注射组织支架。获得了粒径为 ~22 ± 5.3 nm 的 nHA，并且 nHAMC 显示出蜂窝状 3D 微孔结构。Nano-HA 具有比 HA 更好的凝胶强度、物理流变学和生物学特性。它有效地降低了高盐含量，从而降低了MC的胶凝温度。随着纳米 HA 掺入量的增加，性能得到改善。与HAMC相比，盐的添加对nHAMC凝胶化温度的影响更为显着；盐析效应取决于 nHA/HA 含量。生物相容性 nHAMC 有助于充分的细胞粘附和增殖，具有比对照更好的迁移率的更多延伸突起。因此，可以断言纳米定制糖胺聚糖的生物调节作用可用于设计有效的热响应支架。