Zebrafish behavior is influenced by the lateral line hair cells and muscles. Drug-induced behavioral changes can serve as indicators in the evaluation of drug toxicity. The aminoglycoside family of antibiotics comprise a number of agents, including neomycin (NM) and gentamicin (GM). We hypothesized that NM and GM exert different effects on zebrafish larvae through their action on the lateral line and muscle fibers, inducing different swimming behavioral patterns such as locomotor behavior and the startle response. In this study, 125 μM NM and 5, 10, 20 μM GM induced hair cell damage in the anterior and posterior lateral lines of zebrafish larvae. However, unlike GM, 125 μM NM also caused muscle damage. Locomotor behavior was decreased in the 125 μM NM-exposed group compared to the group exposed to GM. Furthermore, 125 μM NM exposure induced significantly different patterns of various indices of startle behavior compared with the GM exposure groups. Additionally, the larvae exhibited different startle responses depending on the concentration of GM. These results suggest that GM may be the drug-of-choice for analyzing behavioral changes in zebrafish caused by damage to the lateral line alone. Our study highlights the importance of confirming muscle damage in behavioral analyses using zebrafish.

中文翻译：

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由氨基糖苷引起的侧线和肌肉损伤引起的斑马鱼幼虫的行为变化分析。

斑马鱼的行为受到侧线毛细胞和肌肉的影响。药物引起的行为变化可以作为评估药物毒性的指标。抗生素的氨基糖苷家族包含许多药剂，包括新霉素（NM）和庆大霉素（GM）。我们假设NM和GM通过它们对侧线和肌肉纤维的作用对斑马鱼的幼虫发挥不同的作用，诱导不同的游泳行为模式，例如运动行为和惊吓反应。在这项研究中，125μMNM和5、10、20μMGM引起斑马鱼幼虫的前侧和后侧线中的毛细胞损伤。但是，与GM不同，125μMNM也引起肌肉损伤。与暴露于GM的组相比，暴露于125μMNM的组的运动行为降低。此外，与GM暴露组相比，125μMNM暴露引起各种惊吓行为指标的模式明显不同。另外，根据GM的浓度，幼虫表现出不同的惊吓反应。这些结果表明，GM可能是分析仅由侧线损伤引起的斑马鱼行为变化的首选药物。我们的研究强调了在使用斑马鱼的行为分析中确认肌肉损伤的重要性。