Electrospun polyacrylonitrile fiber membranes (EPFMs) were coated with multilayer films, assembled using the layer-by-layer (LbL) technique through the alternate deposition of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA), to develop an antithrombogenic drug release membrane for hemodialysis. Methylene blue (MB) and heparin (HEP) were attached to the PAH and PAA multilayers, respectively, as model drug and antithrombogenic agent to investigate the dual functionality of the membranes. The positively (PAH, MB) and negatively (PAA, HEP) charged groups generated a supermolecular polyelectrolyte multilayer film (SPF) capable of loading high amounts of MB and HEP on the EPFMs at appropriate composition. The pH was fixed at 5.5 during assembly to stabilize the SPF. Heavy assembly of the PAH/PAA multilayer occurred at 10 wt% of both MB and HEP with 25 cycles of LbL deposition, and it exhibited long-term release of MB and low release of HEP at pH 7.4 in a circulatory system. The SPF-coated EPFMs also achieved low platelet attachment after 4 h of platelet rich plasma circulation and showed prolonged clotting times including thromboplastin, thrombin, and prothrombin times. Collectively, these observations suggest that SPF-coated EPFMs have great potential for use as hemodialysis membranes with positively charged drug loading.

中文翻译：

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通过用一层一层的超分子聚电解质薄膜覆盖静电纺聚丙烯腈纤维膜来制备生物滤膜。

将静电纺丝的聚丙烯腈纤维膜（EPFM）涂上多层膜，使用逐层（LbL）技术通过交替沉积聚烯丙胺盐酸盐（PAH）和聚丙烯酸（PAA）进行组装，以进行显影用于血液透析的抗血栓药物释放膜。亚甲基蓝（MB）和肝素（HEP）分别作为模型药物和抗血栓形成剂附着在PAH和PAA多层膜上，以研究膜的双重功能。带正电（PAH，MB）和带负电（PAA，HEP）的基团生成了一种超分子聚电解质多层膜（SPF），能够以适当的组成将大量的MB和HEP负载在EPFM上。在组装过程中将pH固定在5.5以稳定SPF。PAH / PAA多层的重组装发生在MB和HEP的10 wt％处，具有25个LbL沉积循环，并且在循环系统中，在pH 7.4下，MB长期释放而HEP释放低。在富含血小板的血浆循环4小时后，涂有SPF的EPFM还实现了低血小板附着，并显示出凝血时间延长，包括凝血活酶，凝血酶和凝血酶原时间。总体而言，这些观察结果表明，涂​​有SPF的EPFM具有巨大的潜力，可以用作载有正电荷药物的血液透析膜。在富含血小板的血浆循环4小时后，涂有SPF的EPFM还实现了低血小板附着，并显示出凝血时间延长，包括凝血活酶，凝血酶和凝血酶原时间。总体而言，这些观察结果表明，涂​​有SPF的EPFM具有巨大的潜力，可以用作载有正电荷药物的血液透析膜。在富含血小板的血浆循环4小时后，涂有SPF的EPFM还实现了低血小板附着，并显示出凝血时间延长，包括凝血活酶，凝血酶和凝血酶原时间。总体而言，这些观察结果表明，涂​​有SPF的EPFM具有巨大的潜力，可以用作载有正电荷药物的血液透析膜。