Recent studies have shown that laboratory murine autoimmunity models under the same environment display different outcomes. We established diabetic nephropathy model mice under the same environment using the classic streptozotocin method. Renal dysfunction was different among the mice. Proteinuria was more significant in the severe proteinuria group (SP) than in the mild proteinuria group (MP). We hypothesized a role for the gut microbiota in the outcome and reproducibility of induced DN models. 16S rDNA gene sequencing technology was used to analyze the differences in the gut microbiota between the two groups. Here, through fecal microbiota transplantation (FMT) and gas chromatography mass spectrometry (GC–MS), we verified the role of the gut microbiota and its short-chain fatty acid (SCFA) generation in DN mouse renal dysfunction. In the SP group, there was a reduced abundance of Firmicutes (P < 0.0001), and the dominant genus Allobaculum [linear discriminant analysis (LDA) >3, P < 0.05] was positively correlated with body weight (Rho = 0.767, P < 0.01) and blood glucose content (Rho = 0.648, P < 0.05), while the dominant genus Anaerosporobacter (LDA > 3, P < 0.05) was positively correlated with 24-hour urinary protein content (Rho = 0.773, P < 0.01). In the MP group, the dominant genus Blautia (LDA > 3, P < 0.05) was negatively correlated with 24-hour urinary protein content (Rho = −0.829, P < 0.05). The results indicated that Allobaculum and Anaerosporobacter may worsen renal function, while Blautia may be a protective factor in DN. These findings suggested that the gut microbiota may contribute to the heterogeneity of the induced response since we observed potential disease-associated microbial taxonomies and correlations with DN.

最近的研究表明，在相同环境下的实验室鼠自身免疫模型显示出不同的结果。我们使用经典的链脲佐菌素方法在相同环境下建立了糖尿病肾病模型小鼠。小鼠的肾功能不全有所不同。重度蛋​​白尿组（SP）的蛋白尿比轻度蛋白尿组（MP）更为显着。我们假设肠道菌群在诱导的DN模型的结果和可重复性中的作用。16S rDNA基因测序技术用于分析两组之间肠道菌群的差异。在这里，通过粪便微生物群移植（FMT）和气相色谱质谱（GC-MS），我们证实了肠道微生物群及其短链脂肪酸（SCFA）的产生在DN小鼠肾功能不全中的作用。P  <0.0001），优势菌[线性判别分析（LDA）> 3，P  <0.05]与体重（Rho = 0.767，P  <0.01）和血糖含量（Rho = 0.648，P  < 0.05），而占优势的厌气菌属（LDA> 3，P <0.05）与24小时尿蛋白含量呈正相关（Rho = 0.773，P <0.01）。在MP组中，优势布鲁氏菌属（LDA> 3，P <0.05）与24小时尿蛋白含量呈负相关（Rho = -0.829，P <0.05）。结果表明，同花异色菌和厌气菌可能会使肾功能恶化，而蓝藻可能是DN的保护因子。这些发现表明，由于我们观察到了潜在的疾病相关微生物分类法以及与DN的相关性，因此肠道菌群可能有助于诱导反应的异质性。