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Transcriptome analysis of HPV-induced warts and healthy skin in humans
BMC Medical Genomics ( IF 2.7 ) Pub Date : 2020-03-09 , DOI: 10.1186/s12920-020-0700-7
Laith N. AL-Eitan , Amneh H. Tarkhan , Mansour A. Alghamdi , Firas A. Al-Qarqaz , Hadeel S. Al-Kofahi

The human papillomaviruses (HPV) are a group of viruses that, depending on the strain, can cause cancer or the formation of benign growths known as warts. Scarce information exists with regard to the genetic nature of non-genital cutaneous warts induced by the human papillomavirus (HPV). The main purpose of this study is to investigate the differences between the gene expression profiles of common warts and healthy skin in HPV-positive individuals by RNA sequencing on the Illumina HiSeq 2500. After obtaining shave biopsies of common warts and healthy skin from twelve Arab males, we were able to analyze the transcriptomes of 24 paired cases and controls. Common warts were found to possess a highly significant and unique molecular signature. Many of the most up-regulated (KRT16, EPGN, and ABCG4) and down-regulated genes (C15orf59, CYB561A3, and FCGRT) in warts were the subject of little investigation in the published literature. Moreover, the top 500 differentially expressed genes were found to be associated with immune and autoimmune pathways, such as the neutrophil degranulation, toll-like receptor 7/8 (TLR 7/8) cascade, toll-like receptor 9 (TLR9) cascade, and toll-like receptor 10 (TLR10) pathways, among others. Our findings are particularly important because they serve as the most comprehensive to date with regard to the modulation of human skin gene expression by HPV infection.

中文翻译:

HPV诱发的疣和人类健康皮肤的转录组分析

人乳头瘤病毒(HPV)是一类病毒,根据病毒株的不同,它们会引起癌症或形成良性生长,称为疣。关于由人乳头瘤病毒(HPV)引起的非生殖器皮肤疣的遗传性质,信息稀少。这项研究的主要目的是通过在Illumina HiSeq 2500上进行RNA测序研究HPV阳性个体中普通疣和健康皮肤的基因表达谱之间的差异。在从十二名阿拉伯男性那里获得了普通疣和健康皮肤的剃须活检后,我们能够分析24个配对病例和对照的转录组。发现普通疣具有高度重要且独特的分子特征。许多上调程度最高的基因(KRT16,EPGN和ABCG4)和下调基因(C15orf59,CYB561A3,疣中的FCGRT和FCGRT)在已发表的文献中很少进行研究。此外,发现前500个差异表达基因与免疫和自身免疫途径相关,例如嗜中性粒细胞脱粒,toll​​样受体7/8(TLR 7/8)级联,toll​​样受体9(TLR9)级联,以及Toll样受体10(TLR10)通路等。我们的发现特别重要,因为它们是迄今为止关于HPV感染调节人类皮肤基因表达的最全面的研究。Toll样受体9(TLR9)级联,以及Toll样受体10(TLR10)通路等。我们的发现特别重要,因为它们是迄今为止关于HPV感染调节人类皮肤基因表达的最全面的研究。Toll样受体9(TLR9)级联,以及Toll样受体10(TLR10)通路等。我们的发现特别重要,因为它们是迄今为止关于HPV感染调节人类皮肤基因表达的最全面的研究。
更新日期:2020-04-22
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