Sepsis represents a complex disease with dysregulated inflammatory response and high mortality rate. Long noncoding RNAs (lncRNAs) have been reported to play regulatory roles in a variety of biological processes. However, studies evaluating the function of lncRNAs in pediatric sepsis are scarce, and current knowledge of the role of lncRNAs in pediatric sepsis is still limited. The present study explored the expression patterns of both lncRNAs and mRNAs between pediatric sepsis patients and healthy controls based on a comprehensive microarray analysis. LncRNA and mRNA microarray was used to detect the expression of lncRNAs and mRNAs in the septic and control groups. Aberrantly expressed mRNAs and lncRNAs identified were further interpreted by enrichment analysis, receiver operating characteristic (ROC) curve analysis, co-expression network analysis, and quantitative real-time PCR (qPCR). A total of 1488 differetially expressed lncRNAs and 1460 differentially expressed mRNAs were identified. A co-expression network of the identified lncRNAs and mRNAs was constructed. In this network, lncRNA lnc-RP11-1220 K2.2.1–7 is correlated with mRNA CXCR1 and CLEC4D; lncRNA lnc-ANXA3–2 is correlated with mRNA CLEC4D; lncRNA lnc-TRAPPC5–1 is correlated with mRNA DYSF and HLX; lncRNA lnc-ZNF638–1 is correlated with mRNA DYSF and HLX. Significantly different expressions between pediatric sepsis patients and controls were validated by qPCR for the 4 lncRNAs and 4 co-expressed mRNAs, validating the microarray results. Our study contributes to a comprehensive understading of the involvment of lncRNAs and mRNAs in pediatric sepsis, which may guide subsequent experimental research. Furthermore, our study may also provide potential candidate lncRNAs and mRNAs for the diagnosis and treatment of pediatric sepsis.

中文翻译：

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小儿败血症中的长非编码RNA和信使RNA异常：初步研究

脓毒症是一种炎症反应失调且死亡率高的复杂疾病。据报道，长非编码RNA（lncRNA）在多种生物学过程中起调节作用。但是，尚缺乏评估lncRNA在小儿败血症中功能的研究，目前对lncRNA在小儿败血症中作用的了解仍然有限。本研究基于全面的微阵列分析探索了小儿败血症患者和健康对照之间lncRNA和mRNA的表达模式。用LncRNA和mRNA微阵列检测脓毒症和对照组中lncRNA和mRNA的表达。通过富集分析，受体工作特征（ROC）曲线分析，共表达网络分析，进一步解释鉴定出的异常表达的mRNA和lncRNA。定量实时PCR（qPCR）。鉴定出总共1488个差异表达的lncRNA和1460个差异表达的mRNA。构建了鉴定出的lncRNA和mRNA的共表达网络。在该网络中，lncRNA lnc-RP11-1220 K2.2.1–7与mRNA CXCR1和CLEC4D相关。lncRNA lnc-ANXA3–2与mRNA CLEC4D相关。lncRNA lnc-TRAPPC5-1与mRNA DYSF和HLX相关。lncRNA lnc-ZNF638-1与DYSF和HLX mRNA相关。通过4个qncRNA和4个共表达的mRNA的qPCR验证了小儿败血症患者和对照之间的显着差异，从而验证了微阵列结果。我们的研究有助于小儿败血症中lncRNA和mRNA参与的全面了解，这可能会指导后续的实验研究。此外，