Phytochemical investigation of the alkaloid extract of the aerial parts of Psychotria nemorosa led to the isolation and characterization of 10 azepine-indole alkaloids, i.e., cimitrypazepine (1), fargesine (2), nemorosines A (3), and B (12), nemorosinosides A-F (4-9), as well as two β-carboline derivatives, 10-hydroxyisodolichantoside (10) and 10-hydroxydolichantoside (11), an isoxazole alkaloid, nemorosinoside G (13), serotonin (14), bufotenine (15), and (S)-gentianol (16). Compounds 3-13 have not yet been described. These compounds were isolated by semipreparative HPLC, and their structures were determined by means of HRMS, NMR, and ECD measurements. In addition, the monoamine oxidase-A (MAO-A), MAO-B, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) inhibitory activities were evaluated. Alkaloids 1-3 inhibited the MAO-A activity with IC50 values of 1.4, 1.4, and 0.9 μM, respectively.

中文翻译：

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Psychotria nemorosa的生物活性氮杂吲哚生物碱。

对Psychotria nemorosa空中部分的生物碱提取物进行植物化学研究后，分离并鉴定了10种氮杂吲哚生物碱，即西米替平（1），fargesine（2），nemorosines A（3）和B（12）， Nemorosinosides AF（4-9）以及两种β-咔啉衍生物，10-hydroxyisodolichantoside（10）和10-hydroxydolichantoside（11），异恶唑生物碱，nemorosinoside G（13），5-羟色胺（14），bufotenine（15） ，和（S）-龙胆酚（16）。尚未描述化合物3-13。通过半制备HPLC分离这些化合物，并通过HRMS，NMR和ECD测量确定其结构。此外，评估了单胺氧化酶-A（MAO-A），MAO-B，乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的抑制活性。