Biodegradable and renewable materials, such as cellulose nanomaterials, have been studied as a replacement material for traditional plastics in the biomedical field. Furthermore, in chronic wound care, modern wound dressings, hydrogels, and active synthetic extracellular matrices promoting tissue regeneration are developed to guide cell growth and differentiation. Cells are guided not only by chemical cues but also through their interaction with the surrounding substrate and its physicochemical properties. Hence, the current work investigated plant-based cellulose nanomaterials and their surface characteristic effects on human dermal fibroblast (HDF) behavior. Four thin cellulose nanomaterial-based coatings produced from microfibrillar cellulose (MFC), cellulose nanocrystals (CNC), and two TEMPO-oxidized cellulose nanofibers (CNF) with different total surface charge were characterized, and HDF viability and adhesion were evaluated. The highest viability and most stable adhesion were on the anionic CNF coating with a surface charge of 1.14 mmol/g. On MFC and CNC coated surfaces, HDFs sedimented but were unable to anchor to the substrate, leading to low viability.

中文翻译：

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人皮肤成纤维细胞的活力和对纤维素纳米材料涂层的附着力：表面特性的影响。

已经研究了生物可降解和可再生材料，例如纤维素纳米材料，作为生物医学领域中传统塑料的替代材料。此外，在慢性伤口护理中，开发了现代伤口敷料，水凝胶和促进组织再生的活性合成细胞外基质，以指导细胞生长和分化。细胞不仅受到化学提示的指导，而且还受到与周围底物的相互作用及其物理化学特性的指导。因此，当前的工作研究了基于植物的纤维素纳米材料及其表面特征对人类皮肤成纤维细胞（HDF）行为的影响。由微纤丝纤维素（MFC），纤维素纳米晶体（CNC），表征了两种具有不同总表面电荷的TEMPO氧化纤维素纳米纤维（CNF），并评估了HDF的活力和附着力。最高的活性和最稳定的附着力是在阴离子CNF涂层上，表面电荷为1.14 mmol / g。在MFC和CNC涂层的表面上，HDF沉淀了，但无法锚固在基材上，从而降低了生存能力。