Evidence of the continuous rise of novel doping agents and novel doping strategies calls for the development of more accurate multi‐target screening methods. Direct multi‐target screening approaches are restricted to the targeted substances and their turnover. The development of effective “indirect” screening methods requires a priori a deep understanding of the metabolism of the substance. The biological passport has been demonstrated to be very effective, but it is limited to about 20 indirect parameters. The standard antidoping analytical methods are hence targeted and do not aim directly to identify unknown substances. Also, the detection of doping agents is limited by the excretion of the substance. This study considers metabolomics for the screening of performance enhancing hormone abuse by athletes, based on the following pieces of evidence: (1) hormones have a strong influence on human metabolism, changing several parameters in many tissues, organs, and bio‐fluids; (2) metabolomics has been demonstrated to be a very accurate tool to depict the metabolic status of several organisms, tissues, and for several human diseases, including hormone deficiencies; (3) metabolomics has been demonstrated to be able to distinguish hormone‐treated animals from controls in many species, without the need for a priori knowledge of the metabolism for the specific substance. The literature shows that metabolomics could be an appropriate tool to detect hormone abuse, keeping in mind the strength and the limitation of such an approach.

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代谢组学在反兴奋剂工具箱中的作用？

新型掺杂剂和新型掺杂策略不断增长的证据要求开发更准确的多目标筛选方法。直接多目标筛选方法仅限于目标物质及其周转率。有效的“间接”筛选方法的发展需要事先对物质的代谢有深刻的了解。生物护照已被证明是非常有效的，但仅限于约20个间接参数。因此，标准的反掺杂分析方法是针对性的，并不直接针对识别未知物质。而且，掺杂剂的检测受到物质排泄的限制。这项研究基于以下证据，考虑了代谢组学技术来筛查运动员增强表现的荷尔蒙滥用：（1）激素对人体的新陈代谢有很大影响，改变了许多组织，器官和生物流体中的几个参数；（2）代谢组学已被证明是描述几种生物，组织以及几种人类疾病（包括激素缺乏症）的代谢状态的非常准确的工具；（3）代谢组学已被证明能够将激素治疗的动物与许多物种的对照区分开来，而无需先验了解特定物质的代谢。文献表明，代谢组学可能是检测激素滥用的合适工具，同时要记住这种方法的优势和局限性。（2）代谢组学已被证明是描述几种生物，组织以及几种人类疾病（包括激素缺乏症）的代谢状态的非常准确的工具；（3）代谢组学已被证明能够将激素治疗的动物与许多物种的对照区分开来，而无需先验了解特定物质的代谢。文献表明，代谢组学可能是检测激素滥用的合适工具，同时要记住这种方法的优势和局限性。（2）代谢组学已被证明是描述几种生物，组织以及几种人类疾病（包括激素缺乏症）的代谢状态的非常准确的工具；（3）代谢组学已被证明能够将激素治疗的动物与许多物种的对照区分开来，而无需先验了解特定物质的代谢。文献表明，代谢组学可能是检测激素滥用的合适工具，同时要记住这种方法的优势和局限性。无需对特定物质的代谢有先验知识。文献表明，代谢组学可能是检测激素滥用的合适工具，同时要记住这种方法的优势和局限性。无需对特定物质的代谢有先验知识。文献表明，代谢组学可能是检测激素滥用的合适工具，同时要记住这种方法的优势和局限性。