The resistance of H‐type and L‐type BSE prions to autoclaving under EU regulation conditions for specified risk material is unknown. We employed transgenic mouse (bovinized line tg1896) bioassay to assess the efficacy of such decontamination on L‐ and H‐type BSE. For each source, titre calculation was based on the comparison of incubation period and attack rate prior to and after decontamination. The infectious titre of L‐type BSE was reduced by at least 9.40 log₁₀ and of H‐type BSE by at least 3.94 log₁₀. In fact, no infectivity was detected for L‐type or H‐type BSE post‐inactivation even at a 10^–1 dilution.

中文翻译：

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推荐的高压灭菌锅消毒程序使H型和L型牛海绵状脑病灭活

在欧盟法规规定的条件下，对于特定风险材料，H型和L型BSE病毒的耐高压灭菌性尚不清楚。我们采用转基因小鼠（bovinized系tg1896）生物测定法评估了这种去污对L型和H型BSE的功效。对于每种来源，效价的计算是基于去污前后潜伏期和侵袭率的比较。L型BSE的感染滴度至少降低了9.40 log ₁₀，H型BSE的感染滴度至少降低了3.94 log ₁₀。实际上，即使稀释^10–1，L型或H型BSE灭活后也没有检测到感染性。