Inonotus obliquus is a traditional mushroom well known for its therapeutic value. In this study, various solvent fractions of I. obliquus were preliminarily screened for their antioxidant, α-amylase and α-glucosidase inhibition properties. To improve the drug delivery, the active fraction (ethyl acetate fraction) of I. obliquus was synthesized into fungisome (ethyl acetate phophotidyl choline complex, EAPC) and its physical parameters were assessed using Fourier transform infrared spectroscopy (FTIR), High performance liquid chromatography (HPLC), Scanning electron microscope (SEM), and ς potential analysis. Then normal human hepatic L02 cells was used to evaluate the cytotoxicity of EAPC. The results showed that EA fraction possesses significant free radical scavenging, α-amylase and α-glucosidase inhibition properties. FTIR, SEM, and HPLC analysis confirmed the fungisome formation. The particle size of EAPC was 102.80 ± 0.42 nm and the ς potential was -54.30 ± 0.61 mV. The percentage of drug entrapment efficiency was 97.13% and the drug release rates of EAPC in simulated gastric fluid and simulated intestinal fluid were 75.04 ± 0.29% and 93.03 ± 0.36%, respectively. EAPC was nontoxic to L02 cells, however it could selectively fight against the H2 O2 induced oxidative damage in L02 cells. This is the first study to provide scientific information to utilize the active fraction of I. obliquus as fungisome. PRACTICAL APPLICATIONS: Inonotus obliquus (IO) is a traditional medicinal fungus. The extracts of IO have obvious antioxidant and hypoglycemic activities. Ethyl acetate (EA) fraction of IO was encapsulated in liposomes to form EAPC. EAPC has a sustained-release effect. It has nontoxic to L02 cells and could protect L02 cells from oxidative damage caused by hydrogen peroxide. This study could provide new ideas for the treatment of diabetes.

中文翻译：

---

I. obliquus 各种溶剂组分的抗氧化、α-淀粉酶和α-葡萄糖苷酶活性以及活性组分对 H 2 O 2 诱导的肝 L02 细胞损伤的预防作用作为真菌

Ininotusobliquus 是一种传统的蘑菇，以其治疗价值而闻名。在这项研究中，初步筛选了 I. obliquus 的各种溶剂级分，以了解它们的抗氧化、α-淀粉酶和 α-葡萄糖苷酶抑制特性。为改善药物递送，将斜交菌的活性组分（乙酸乙酯组分）合成为真菌体（乙酸乙酯磷脂酰胆碱复合物，EAPC），并使用傅里叶变换红外光谱（FTIR）、高效液相色谱法评估其物理参数(HPLC)、扫描电子显微镜 (SEM) 和 ς 电位分析。然后使用正常人肝L02细胞评价EAPC的细胞毒性。结果表明，EA组分具有显着的自由基清除、α-淀粉酶和α-葡萄糖苷酶抑制特性。红外光谱、扫描电镜、和 HPLC 分析证实了真菌体的形成。EAPC 的粒径为 102.80 ± 0.42 nm 且 ς 电位为 -54.30 ± 0.61 mV。EAPC在模拟胃液和模拟肠液中的药物包封率为97.13%，药物释放率分别为75.04±0.29%和93.03±0.36%。EAPC 对 L02 细胞无毒，但它可以选择性地对抗 H2 O2 诱导的 L02 细胞氧化损伤。这是第一项提供科学信息的研究，以利用 I. obliquus 的活性部分作为真菌。实际应用： Inonotus Obliquus (IO) 是一种传统的药用真菌。IO提取物具有明显的抗氧化和降血糖活性。IO 的乙酸乙酯 (EA) 部分被封装在脂质体中以形成 EAPC。EAPC 具有缓释作用。对L02细胞无毒，可保护L02细胞免受过氧化氢引起的氧化损伤。该研究可为糖尿病的治疗提供新思路。