BcIEB is a small protein secreted by the phytopathogenic fungus Botrytis cinerea that is recognized as a pathogen‐associated molecular pattern (PAMP) by plants. This activity is mapped to a highly conserved region of 35 amino acids, the peptide ieb35. Moreover, it has been shown that the protein and the peptide induce systemic resistance to B. cinerea after their infiltration into tobacco leaves. In this work, the possible use of BcIBE1 or ieb35 as plant protective agents has been tested. Tobacco plants were sprayed, infiltrated, or treated at the seedling stage with the protein or the peptide, and plant susceptibility to pathogens with different lifestyles was then studied at various times after treatment. The results showed that both BcIEB1 and ieb35 caused a similar reduction in the lesion sizes caused by B. cinerea and in colonization by Pseudomonas syringae pv. tabaci (reduced by c. 30% and c. 40%, respectively), independently of the treatment type. In addition, the number of B. cinerea inoculations leading to successful infections was also reduced in plants infiltrated or sprayed with BcIEB1/ieb35. The defence‐related genes PR1a, NPR1, and osmotin ap24 were all induced by the protein and the peptide. BcIEB1, and especially ieb35, may be considered as a potential environmentally friendly treatment to increase plant resistance to pathogens.

中文翻译：

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激发子蛋白BcIEB1和衍生肽ieb35提供长期植物保护

BcIEB是一种植物病原性真菌灰葡萄孢所分泌的一种小蛋白，被植物识别为病原体相关分子模式（PAMP）。该活性被定位到35个氨基酸的高度保守的区域，即肽ieb35。而且，已经表明，蛋白质和肽诱导对B的系统抗性。 灰质它们渗入烟叶后。在这项工作中，已经测试了BcIBE1或ieb35作为植物保护剂的可能用途。在苗期对烟草植物进行喷雾，浸润或处理，然后使用蛋白质或多肽，然后在处理后的不同时间研究植物对不同生活方式病原体的敏感性。结果表明，BcIEB1和ieb35均引起由B引起的病变大小的相似减少。 灰霉病并在丁香假单胞菌PV中定殖。烟酸（分别降低约30％和约40％），与治疗类型无关。另外，B的数量。 灰质在用BcIEB1 / ieb35浸润或喷雾的植物中，成功感染的接种量也减少了。与防御相关的基因PR1a，NPR1和osmotin ap24均由蛋白质和肽诱导。BcIEB1，尤其是ieb35，可以被视为增加植物对病原体抗性的潜在环境友好型处理方法。