As an important edible traditional Chinese medicine, Codonopsis pilosula has good immunomodulation effects. This study focuses on C. pilosula oligosaccharides (CPO), which are the sweetness components of C. pilosula. CPO were obtained through systematic separation and purification (the yield is 14.3%), and the effect of CPO on the immunological activities of immunocompromised mice induced by cyclophosphamide (CTX) was evaluated. The results showed that CPO could increase immune organ indices, phagocytic index and immunoglobulin contents, stimulate the proliferation of splenic lymphocytes (coordinating with ConA and LPS), enhance the earlap swelling of the DTH reaction, promote the production of NO and cytokines (IL-2 and IFN-γ) and upregulate the expression of the corresponding mRNA. In addition, CPO upregulated the protein expression of phosphorylated p38, phosphorylated ERK1/2 and phosphorylated JNK, which indicated that CPO might exert immunomodulatory effects through the MAPK signaling pathway. These findings indicated that CPO are important immunomodulatory components in C. pilosula and could be developed as immunomodulators in medicine or functional food areas.

中文翻译：

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党参党参寡糖对小鼠环磷酰胺诱导的免疫抑制的免疫增强作用。

党参是重要的食用中药，具有良好的免疫调节作用。这项研究的重点是C. pilosula寡糖（CPO），它是C. pilosula的甜味成分。通过系统分离和纯化获得CPO（收率为14.3％），并评价了CPO对环磷酰胺（CTX）诱导的免疫受损小鼠免疫活性的影响。结果表明CPO可以增加免疫器官指数，吞噬指数和免疫球蛋白含量，刺激脾淋巴细胞的增殖（与ConA和LPS配合），增强DTH反应的耳廓肿胀，促进NO和细胞因子（IL- 2和IFN-γ），并上调相应mRNA的表达。此外，CPO上调磷酸化p38，ERK1 / 2和JNK磷酸化的蛋白表达，提示CPO可能通过MAPK信号通路发挥免疫调节作用。这些发现表明，CPO是毛状弯曲杆菌中重要的免疫调节成分，可以在医学或功能食品领域用作免疫调节剂。