Polycaprolactone (PCL) is considered as a most widely used biodegradable polymers in tissue engineering. But, PCL is also associated with certain limitations like, low stiffness, hydrophobic nature and limited cell affinity. These drawbacks are addressed in the present study by incorporating different wt% of silicon dioxide (SiO₂) and hydroxyapatite (HAp) in the PCL matrix. 3D scaffolds were developed using a novel BioExtruder. The physicochemical properties, thermal stability and wettability of the composite scaffolds were studied systematically. Optical and Scanning Electron Microscopic images were analysed for morphological evaluation of the scaffolds. The pore size of the developed scaffolds increased from 290 to 315 μm with increasing SiO₂ content, as examined by scanning electron microscope. An improved compressive modulus of 68.82 MPa was observed for 15 wt% SiO₂ incorporated composite scaffold. The in-vitro degradation study of the composite scaffolds demonstrated an increase in the degradation rate for PCL/HAp scaffolds, while no significant change was observed for SiO₂ incorporated scaffolds. Further, the cytotoxicity and cell proliferation studies were carried out using L929 Mouse Fibroblasts and MG-63 Osteoblasts respectively. The developed scaffolds revealed no toxic effects towards the cellular response and an increase in cell proliferation of ≥90% was observed during 7 days of cell culture. Thus, the scaffolds were proved to be potential candidate for bone tissue engineering application, particularly the scaffold with 10 wt% SiO₂ incorporation into PCL/HAp (75/15) composite has resulted into higher cell proliferative % and improved mechanical strength.

聚己内酯（PCL）被认为是组织工程中使用最广泛的生物可降解聚合物。但是，PCL还与某些局限性相关，例如低刚度，疏水性和有限的细胞亲和力。通过在PCL基质中掺入不同重量百分比的二氧化硅（SiO ₂）和羟基磷灰石（HAp），可以解决本研究中的这些缺点。使用新型BioExtruder开发了3D支架。系统地研究了复合支架的理化性质，热稳定性和润湿性。分析光学和扫描电子显微镜图像以对支架进行形态评估。随着SiO _2的增加，发达的支架的孔径从290μm增加到315μm含量，通过扫描电子显微镜检查。对于掺入15wt％的SiO _2的复合支架，观察到68.82MPa的改进的压缩模量。复合支架的体外降解研究表明，PCL / HAp支架的降解速率增加，而SiO ₂没有观察到明显变化。合并的脚手架。此外，分别使用L929小鼠成纤维细胞和MG-63成骨细胞进行了细胞毒性和细胞增殖研究。发达的支架没有显示出对细胞反应的毒性作用，并且在细胞培养7天中观察到细胞增殖的增加≥90％。因此，该支架被证明是骨组织工程应用的潜在候选者，特别是将具有10 wt％SiO ₂掺入PCL / HAp（75/15）复合材料中的支架产生了更高的细胞增殖百分比并提高了机械强度。