St. Paul’s Wort (Siegesbeckia orientalis L.) confers anti-oxidative, anti-inflammatory, anti-allergic, anti-infertility, and immunosuppressive properties. Here, we elucidated whether high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) had anti-adipogenic activity. SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression of adipogenic regulatory factors [peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE decreased the mRNA expression levels of lipogenic enzymes (fatty acid synthase and acetyl-CoA carboxylase) as well as adipocytokines (adiponectin and leptin). The inhibitory effect of SPW-HHPE on adipogenesis was mainly attributed to the enhancement of the Wnt/β-catenin signaling pathway. When β-catenin siRNA was transfected into 3T3-L1 adipocytes, the mRNA expression of PPARγ and C/EBPα was upregulated; however, their expression was attenuated by SPW-HHPE. These results suggest that SPW-HHPE suppresses adipogenesis by stimulating Wnt/β-catenin pathway.

圣保罗草（Siegesbeckia OrientalisL.）具有抗氧化，抗炎，抗过敏，抗不育和免疫抑制的特性。在这里，我们阐明了圣保罗草汁的高静水压提取物（SPW-HHPE）是否具有抗脂肪形成活性。SPW-HHPE通过减少细胞内脂质积累来抑制脂肪形成。SPW-HHPE降低了脂肪形成调节因子[过氧化物酶体增殖物激活受体γ（PPARγ），CCAAT /增强子结合蛋白α（C /EBPα）和固醇调节因子结合蛋白-1c]的mRNA和蛋白表达。此外，SPW-HHPE降低了脂肪生成酶（脂肪酸合酶和乙酰辅酶A羧化酶）以及脂肪细胞因子（脂联素和瘦素）的mRNA表达水平。SPW-HHPE对脂肪形成的抑制作用主要归因于Wnt /β-catenin信号通路的增强。当将β-cateninsiRNA转染到3T3-L1脂肪细胞中时，PPARγ和C /EBPα的mRNA表达增加。然而，它们的表达被SPW-HHPE减弱。这些结果表明，SPW-HHPE通过刺激Wnt /β-catenin途径抑制脂肪形成。