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Discriminative expression of CD39 and CD73 in Cerebrospinal fluid of patients with Multiple Sclerosis and Neuro-Behçet’s disease
Cytokine ( IF 3.8 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.cyto.2020.155054
Khadija Bahrini 1 , Meriam Belghith 1 , Olfa Maghrebi 1 , Jihène Bekir 1 , Mariem Kchaou 2 , Cyrine Jeridi 3 , Rim Amouri 3 , Faycel Hentati 3 , Samir Belal 4 , Samia Ben Sassi 3 , Mohamed-Ridha Barbouche 5
Affiliation  

Treg-mediated immune suppression involves many molecular mechanisms including the cleavage of inflammatory extracellular ATP to adenosine by CD39 ectoenzyme. In the peripheral blood of Multiple Sclerosis (MS) patients, it has been suggested that CD39+ Treg cells have the potential to suppress pro-inflammatory IL-17 secreting cells. Herein, we studied cellular phenotype and mRNA expression of CD39 and CD73 ectoenzymes in the Cerebrospinal fluid (CSF) of MS patients and another neuro-inflammatory disease: the Neuro-behçet's disease (NBD). Using qRT-PCR, we assessed mRNA expression of CD39 and CD73 as well as anti-inflammatory (IL-10) and pro-inflammatory (IL-6, TNF-α, IL-1β) cytokines in patients Peripheral blood mononuclear cells (PBMCs) and CSF of 28 relapsing-remitting multiple sclerosis (RRMS), 20 NBD and 22 controls with non inflammatory neurological disorders (NIND). The most substantial result in the CSF was the higher expression of CD39 in both RRMS and NBD patients compared to NIND. While, the expression of CD73 in CSF samples of NBD was low. In RRMS samples, we detected a significant positive correlation of both CD39 and CD73 with IL-10 expression. Moreover, results by flow cytometry revealed a high percentage of CD39 Treg cells in RRMS CSF. CD39 was preferentially expressed on B cells of NBD. Regarding inflammatory response, we showed a significant increase of IL-6 mRNA expression in NBD patients CSF while in RRMS this increase concerned TNF-α. These results bring evidence that CD39 correlates positively with an anti-inflammatory IL-10 response in RRMS. In contrast, no such association was observed in CSF of NBD patients and CD39 was preferentially expressed on B cells.

中文翻译:

多发性硬化和神经白塞病患者脑脊液中CD39和CD73的鉴别表达

Treg 介导的免疫抑制涉及许多分子机制,包括 CD39 胞外酶将炎性细胞外 ATP 裂解为腺苷。在多发性硬化症 (MS) 患者的外周血中,有人提出 CD39+ Treg 细胞具有抑制促炎性 IL-17 分泌细胞的潜力。在此,我们研究了 MS 患者脑脊液 (CSF) 和另一种神经炎症疾病:神经白塞病 (NBD) 中 CD39 和 CD73 胞外酶的细胞表型和 mRNA 表达。使用 qRT-PCR,我们评估了患者外周血单核细胞 (PBMC) 中 CD39 和 CD73 以及抗炎 (IL-10) 和促炎 (IL-6、TNF-α、IL-1β) 细胞因子的 mRNA 表达) 和 28 例复发缓解型多发性硬化症 (RRMS) 的脑脊液,20 名 NBD 和 22 名患有非炎症性神经系统疾病 (NIND) 的对照。CSF 中最重要的结果是与 NIND 相比,RRMS 和 NBD 患者中 CD39 的表达更高。而NBD的CSF样本中CD73的表达较低。在 RRMS 样本中,我们检测到 CD39 和 CD73 与 IL-10 表达显着正相关。此外,流式细胞术的结果显示 RRMS CSF 中 CD39 Treg 细胞的百分比很高。CD39 优先在 NBD 的 B 细胞上表达。关于炎症反应,我们发现 NBD 患者 CSF 中 IL-6 mRNA 表达显着增加,而在 RRMS 中,这种增加与 TNF-α 有关。这些结果证明 CD39 与 RRMS 中的抗炎 IL-10 反应呈正相关。相比之下,
更新日期:2020-06-01
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