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Analysing the nanoparticle-protein corona for potential molecular target identification.
Journal of Controlled Release ( IF 10.8 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.jconrel.2020.03.008
Chandra Kumar Elechalawar 1 , Md Nazir Hossen 1 , Lacey McNally 2 , Resham Bhattacharya 3 , Priyabrata Mukherjee 4
Affiliation  

When nanoparticles are introduced into biological systems, host proteins tend to associate on the particle surface to form a protein layer termed the "protein corona" (PC). Identifying the proteins that constitute the PC can yield useful information about nanoparticle processing, bio-distribution, toxicity and clearance. Similarly, characterizing and identifying proteins within the PC from patient samples provides opportunities to probe disease proteomes and identify molecules that influence the disease process. Thus, nanoparticles represent unique probing tools for discovery of molecular targets for diseases. Here, we report a first review on target identification using nanoparticles in biological samples based on analysing physico chemical interactions. We also summarize the evolution of the PC surrounding various nano-systems, comment on PC signature, address PC complexity in fluids, and outline challenges associated with analysing the PC. In addition, the influence on PC formation of various nanoparticle parameters is summarized; nanoparticle characteristics considered include size, charge, temperature, and surface modifications for both organic and inorganic nanomaterials. We also discuss the advantages of nanotechnology, over other more invasive and laborious methods, for identifying potential diagnostic and therapeutic targets.

中文翻译:

分析纳米颗粒-蛋白质冠以识别潜在的分子靶标。

当纳米颗粒被引入生物系统时,宿主蛋白倾向于在颗粒表面结合形成称为“蛋白冠”(PC)的蛋白质层。识别构成 PC 的蛋白质可以产生有关纳米颗粒加工、生物分布、毒性和清除的有用信息。同样,从患者样本中表征和识别 PC 内的蛋白质为探测疾病蛋白质组和识别影响疾病过程的分子提供了机会。因此,纳米颗粒代表了发现疾病分子靶点的独特探测工具。在这里,我们报告了基于分析物理化学相互作用在生物样品中使用纳米颗粒进行目标识别的首次综述。我们还总结了 PC 围绕各种纳米系统的演变,评论了 PC 特征,解决了 PC 在流体中的复杂性,并概述了与分析 PC 相关的挑战。此外,还总结了各种纳米粒子参数对PC形成的影响;考虑的纳米颗粒特性包括有机和无机纳米材料的尺寸、电荷、温度和表面修饰。我们还讨论了纳米技术相对于其他更具侵入性和费力的方法在识别潜在诊断和治疗靶点方面的优势。
更新日期:2020-03-09
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