Alzheimer’s disease (AD) is the most prevalent type of dementia affecting older people. The identification of biomarkers is increasingly important and would be crucial for future therapy. Here, we demonstrated that in AD erythrocytes: i) the anion transporter band3 is highly phosphorylated; ii) the lyn kinase is phosphorylated and activated; iii) the tyrosine phosphatase activity is downregulated, with a significant inverse correlation between band3 phosphorylation and disease progression, as revealed by Mini Mental State Examination score. Finally, we showed that in normal erythrocytes, treated in vitro with Aβ1−42 peptide, both band3 phosphorylation and lyn activation occurs. These results suggest that modulation of tyrosine phosphorylation signaling may be evaluated as a potential peripheral marker in AD.

阿尔茨海默氏病（AD）是影响老年人的最普遍的痴呆类型。生物标志物的鉴定越来越重要，对于将来的治疗至关重要。在这里，我们证明了在AD红细胞中：i）阴离子转运蛋白带3被高度磷酸化；ii）lyn激酶被磷酸化并被激活；iii）酪氨酸磷酸酶活性被下调，band3磷酸化与疾病进展之间存在显着的逆相关性，如迷你精神状态检查评分所示。最后，我们表明，在正常的红细胞中，用Aβ1-42肽进行体外处理时，band3磷酸化和lyn激活均发生。这些结果表明，酪氨酸磷酸化信号的调节可被评估为AD中潜在的外周标志物。