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Effect of phosphorus amendments on rice rhizospheric methanogens and methanotrophs in a phosphorus deficient soil
Geoderma ( IF 6.1 ) Pub Date : 2020-06-01 , DOI: 10.1016/j.geoderma.2020.114312
Dandan Gao , Rong Sheng , Andrew S. Whiteley , Benjamin Moreira-Grez , Hongling Qin , Wenzhao Zhang , Yu Zhan , Wenxue Wei

Abstract Methane emission can be affected by soil fertility, but little is known about the influence of soil phosphorus (P) nutrition on methane production and oxidation processes. In this study, a pot experiment was conducted to explore the effects of P amendments on activity, abundance and community composition of soil methanogens and methanotrophs using P deficient paddy soil. The abundance and community composition of methanogens and methanotrophs, methane production and oxidation potentials were investigated by real-time quantitative PCR, Miseq high-throughput sequencing and biochemical assays, respectively. The results showed that although P amendments induced increases of both methanogenic and methanotrophic abundances and potential activities within bulk soils, the increases were relatively small. In contrast, P applications resulted in large and significant increases in the copy numbers and potential activity of methanogens within rhizosphere soils, whereas, this phenomenon was not detected for methanotrophs. Moreover, the methanogenic community structure remained similar amongst the P amendment treatments for both rhizosphere and bulk soils. For methanotrophs, P application induced clear shifts in the community composition within rhizosphere soils but not in bulk soils, where the relative abundance of type I methanotrophs increased whilst type II decreased. Our data indicate that the application of phosphate to P deficient paddy soils could preferentially stimulate the abundance and activity of methanogens over methanotrophs within the rice rhizosphere. Ultimately, improving plant growth by P input may accelerate methane emissions through stimulating rhizosphere methanogenic populations without a concomitant increase in the methanotrophic compartment.

中文翻译:

磷肥对缺磷土壤水稻根际产甲烷菌和甲烷氧化菌的影响

摘要 土壤肥力会影响甲烷排放,但土壤磷(P)营养对甲烷产生和氧化过程的影响知之甚少。在这项研究中,进行了盆栽试验,以探索磷添加物对土壤产甲烷菌和甲烷氧化菌的活性、丰度和群落组成的影响,使用磷缺乏的稻田。产甲烷菌和甲烷氧化菌的丰度和群落组成、甲烷产生和氧化电位分别通过实时定量 PCR、Miseq 高通量测序和生化分析进行了研究。结果表明,虽然磷添加物引起了大块土壤中产甲烷和甲烷氧化丰度和潜在活动的增加,但增加相对较小。相比之下,施磷导致根际土壤中产甲烷菌的拷贝数和潜在活性的大量显着增加,而甲烷氧化菌未检测到这种现象。此外,在根际和大块土壤的磷修正处理中,产甲烷群落结构保持相似。对于甲烷氧化菌,施磷导致根际土壤群落组成发生明显变化,但在大块土壤中没有,其中 I 型甲烷氧化菌的相对丰度增加,而 II 型甲烷氧化菌的相对丰度降低。我们的数据表明,在缺磷的稻田土壤中施用磷酸盐可以优先刺激水稻根际内产甲烷菌的丰度和活性,而不是甲烷氧化菌。最终,
更新日期:2020-06-01
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