In recent years, Zn-based materials provide a new option as biodegradable metals for orthopedic applications. To improve the low strength and brittle nature of pure Zn, small amounts of alloying element Mn (0.1, 0.4 and 0.8 wt.%) were added into Zn to fabricate binary Zn-Mn alloys. An extremely high elongation (83.96 ± 2.36%) was achieved in the resulting Zn-0.8 wt.%Mn alloy. Moreover, Zn-Mn alloys displayed significantly improved cytocompatibility as compared to pure Zn, according to cell proliferation and morphology analyses. More importantly, a significantly improved osteogenic activity was verified after adding Mn regarding ALP activity and osteogenic expression. Furthermore, Zn-0.8 wt.%Mn alloy scaffolds were implanted into the rat femoral condyle for repairing bone defects with pure Ti as control. Enhanced osteogenic activities were confirmed for Zn-0.8Mn alloy in contrast to pure Ti based on Micro-CT and histological results, and favorable in vivo biosafety of Zn-0.8Mn alloy was verified by H&E staining and blood tests. The exceptional mechanical performance and favorable osteogenic capability render Zn-Mn alloy a promising candidate material in the treatment of bone defects or fracture repair. STATEMENT OF SIGNIFICANCE: The element Mn, on the one hand, as an essential trace element in the human body, promotes cell proliferation, adhesion, spreading, and regulates bone metabolism; on the other hand, it could significantly improve the ductility of Zn alloys. Here, we systematically reported the biocompatibility and biofunctionality of binary biodegradable Zn-Mn alloys in the bone environment. The Zn-Mn alloys promoted MC3T3-E1 cell proliferation, adhesion, spreading, and osteogenic differentiation in vitro. Furthermore, a rat femoral condyle defect model was established; porous Zn-Mn alloy scaffolds were manufactured to repair the bone defects. Significant bone regenerations, considerable bone ingrowth, and desirable biosafety were confirmed in vivo. Therefore, biodegradable Zn-Mn with promising osteogenic properties may become new options for orthopedic implant materials.

中文翻译：

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设计用于骨科应用的Zn-Mn可生物降解金属的体外和体内研究。

近年来，基于锌的材料为骨科应用提供了可生物降解金属的新选择。为了改善纯锌的低强度和脆性，将少量合金元素Mn（0.1、0.4和0.8 wt。％）添加到Zn中以制造二元Zn-Mn合金。在所得的Zn-0.8wt。％Mn合金中获得了极高的伸长率（83.96±2.36％）。此外，根据细胞增殖和形态分析，与纯锌相比，锌锰合金显示出显着改善的细胞相容性。更重要的是，在添加关于ALP活性和成骨表达的Mn后，证实了显着改善的成骨活性。此外，将Zn-0.8 wt。％Mn合金支架植入大鼠股骨con中，以纯钛为对照修复骨缺损。根据Micro-CT和组织学结果，证实了与纯Ti相比，Zn-0.8Mn合金具有更高的成骨活性，并且通过H＆E染色和血液测试证实了Zn-0.8Mn合金具有良好的体内生物安全性。出色的机械性能和良好的成骨能力使Zn-Mn合金成为治疗骨缺损或骨折修复的有前途的候选材料。重要性说明：一方面，锰元素是人体必需的微量元素，可促进细胞增殖，粘附，扩散并调节骨代谢。另一方面，它可以显着提高锌合金的延展性。在这里，我们系统地报道了骨环境中二元可生物降解的Zn-Mn合金的生物相容性和生物功能。锌锰合金在体外促进MC3T3-E1细胞增殖，粘附，扩散和成骨分化。建立大鼠股骨fe突缺损模型。制造多孔Zn-Mn合金支架以修复骨缺损。在体内证实了显着的骨再生，显着的骨向内生长和合乎需要的生物安全性。因此，具有良好成骨特性的可生物降解的Zn-Mn可能会成为整形外科植入材料的新选择。