Abstract A new method was introduced for determination of absorptivity and subsequent determination of concentration and size of silver nanoparticles (AgNPs). Without using any complicated instrument, based on a kinetic reaction of AgNPs, a ratio curve is extracted and chemometrically fitted to obtain the absorptivity of the AgNPs. Afterwards, it can be possible to estimate size of the AgNPs based on the reported relations. The method can be simply used for any NPs provided that it can absorb in UV–Vis region of the electromagnetic spectrum and after employing in a kinetic reaction. The agreement between the calculated diameter of the AgNPs based on the absorptivity derived from the method and the transmission electron microscopy (TEM) imaging were excellent. It can be claimed that this is the most direct method to determine NPs absorptivity and concentration.

中文翻译：

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银纳米粒子的吸收率和浓度的简单化学计量学估计

摘要 介绍了一种用于测定吸收率以及随后测定银纳米颗粒 (AgNPs) 浓度和尺寸的新方法。在不使用任何复杂仪器的情况下，基于 AgNPs 的动力学反应，提取比率曲线并进行化学计量拟合以获得 AgNPs 的吸收率。之后，可以根据报告的关系估计 AgNPs 的大小。该方法可以简单地用于任何 NP，只要它可以在电磁光谱的 UV-Vis 区域吸收，并且在用于动力学反应后。基于该方法得出的吸收率计算出的 AgNPs 直径与透射电子显微镜 (TEM) 成像之间的一致性非常好。